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<p>Acetylation is one of the ubiquitous modifications of cell wall polysaccharides, which affects cell wall structure and function. Acetyltransferase and acetyl esterase mediate the process of polysaccharides acetylation and are responsible for the dynamic modification of acetyl. Currently, three classes of acetyltransferases (reduced wall acetylation 2, RWA2; trichome birefringence-like, TBL; altered xyloglucan 9, AXY9) and two classes of acetyl esterases (pectin acetylesterase, PAE; brittle leaf sheath 1, BS1) have been identified. Despite the established importance of TBL-mediated acetylation in plant cell wall integrity and immunity in several species (e.g., <i>Arabidopsis</i>, rose, and rice), the <i>ZmTBLs</i> family members implicated in immune responses in maize remain largely unexplored in terms of their genomic distribution, functional diversification, and mechanistic roles. In this study, we identified 66 <i>ZmTBLs</i>, which shared high sequence and structural similarity and not inclined to have non-synonymous substitution. Nearly all ZmTBLs harbor a PC-Esterase domain and conserved GDS- and DxxH-related motifs, with the additional presence of a DCxH motif suggesting a higher level of sequence conservation within the ZmTBLs family. According to the transcriptome data, some <i>ZmTBLs</i> may participate in resistance to multiple pathogens. RT-qPCR showed <i>ZmTBL20</i> was highly induced during necrotrophic pathogen <i>Bipolaris maydis</i> infection, and inoculation assay further validated the function of <i>ZmTBL20</i> as a positive regulator in maize response to <i>B. maydis</i>. Moreover, this modified resistance was achieved by changing the cell wall acetylation levels. These results suggest that <i>TBLs</i> may play a significant role in maintaining resistance to southern corn leaf blight in maize.</p>

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Identification of xylan acetylation-related trichome birefringence-like family genes reveals the roles of ZmTBL20 in resistance to southern corn leaf blight

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年5月26日

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  • Chaotian Liu,
  • Ying Ding,
  • Hongliang Wu,
  • Na Ning,
  • Haiyue Yu,
  • Ruoya Lv,
  • Shu Li,
  • Junjie Hao,
  • Xintao Wang,
  • Xin Xie,
  • Xuecai Zhang,
  • Wende Liu,
  • Zhiqiang Li

摘要

Acetylation is one of the ubiquitous modifications of cell wall polysaccharides, which affects cell wall structure and function. Acetyltransferase and acetyl esterase mediate the process of polysaccharides acetylation and are responsible for the dynamic modification of acetyl. Currently, three classes of acetyltransferases (reduced wall acetylation 2, RWA2; trichome birefringence-like, TBL; altered xyloglucan 9, AXY9) and two classes of acetyl esterases (pectin acetylesterase, PAE; brittle leaf sheath 1, BS1) have been identified. Despite the established importance of TBL-mediated acetylation in plant cell wall integrity and immunity in several species (e.g., Arabidopsis, rose, and rice), the ZmTBLs family members implicated in immune responses in maize remain largely unexplored in terms of their genomic distribution, functional diversification, and mechanistic roles. In this study, we identified 66 ZmTBLs, which shared high sequence and structural similarity and not inclined to have non-synonymous substitution. Nearly all ZmTBLs harbor a PC-Esterase domain and conserved GDS- and DxxH-related motifs, with the additional presence of a DCxH motif suggesting a higher level of sequence conservation within the ZmTBLs family. According to the transcriptome data, some ZmTBLs may participate in resistance to multiple pathogens. RT-qPCR showed ZmTBL20 was highly induced during necrotrophic pathogen Bipolaris maydis infection, and inoculation assay further validated the function of ZmTBL20 as a positive regulator in maize response to B. maydis. Moreover, this modified resistance was achieved by changing the cell wall acetylation levels. These results suggest that TBLs may play a significant role in maintaining resistance to southern corn leaf blight in maize.