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<p>The fungal pathogen <i>Botrytis cinerea</i> causes gray mold disease, with <i>Bcras1</i> serving as a key virulence factor. However, the role of <i>Bcras1</i> in the environmental responses and its downstream signaling pathways remains unclear. This study investigated the function of <i>Bcras1</i> and its regulatory network using gene deletion and transcriptomic analysis. Results showed that Δ<i>Bcras1</i> mutants exhibited severe defects in conidiation, sclerotia formation, acid production, and virulence, along with increased sensitivity to stress and enhanced melanin production. Transcriptome profiling revealed 2236 downregulated and 2149 upregulated genes in Δ<i>Bcras1</i>, including decreased expression of conidiation-related genes (<i>Bcbck1</i>, <i>Bos5</i>, and <i>Bcnop53</i>), sclerotia-related gene (<i>Bcatf1</i>), and MAPK pathway-related genes (<i>Bcsak1</i>, <i>Bcmkk1</i>, <i>Bcbck1</i>, and <i>Bcste50</i>), as well as altered ROS-related genes and upregulated melanin biosynthesis genes (<i>Bcsmr1</i> and <i>Bcscd1</i>). Functional characterization of the downregulated MAPK gene <i>Bcste50</i> showed that it similarly affects growth, development, and virulence. Our results demonstrate that <i>Bcras1</i> regulates multiple pathways controlling fungal development, virulence, and stress adaptation, with <i>Bcste50</i> acting as a potential downstream component in this regulatory network.</p>

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Bcras1 is involved in the regulation of environmental responses and the MAPK pathway in Botrytis cinerea

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年12月5日

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  • Wanyu Zhang,
  • Xuemei Shen,
  • Hua Li,
  • Honghua Li

摘要

The fungal pathogen Botrytis cinerea causes gray mold disease, with Bcras1 serving as a key virulence factor. However, the role of Bcras1 in the environmental responses and its downstream signaling pathways remains unclear. This study investigated the function of Bcras1 and its regulatory network using gene deletion and transcriptomic analysis. Results showed that ΔBcras1 mutants exhibited severe defects in conidiation, sclerotia formation, acid production, and virulence, along with increased sensitivity to stress and enhanced melanin production. Transcriptome profiling revealed 2236 downregulated and 2149 upregulated genes in ΔBcras1, including decreased expression of conidiation-related genes (Bcbck1, Bos5, and Bcnop53), sclerotia-related gene (Bcatf1), and MAPK pathway-related genes (Bcsak1, Bcmkk1, Bcbck1, and Bcste50), as well as altered ROS-related genes and upregulated melanin biosynthesis genes (Bcsmr1 and Bcscd1). Functional characterization of the downregulated MAPK gene Bcste50 showed that it similarly affects growth, development, and virulence. Our results demonstrate that Bcras1 regulates multiple pathways controlling fungal development, virulence, and stress adaptation, with Bcste50 acting as a potential downstream component in this regulatory network.