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<p>Foxtail millet (<i>Setaria italica</i> L.) is an important cereal crop, resilient to abiotic stresses, but suffers significant yield losses from blast disease, caused by <i>Pyricularia grisea</i>. Understanding the genetic mechanisms underlying blast resistance is essential for developing resistant cultivars. This study employed bulk segregant analysis sequencing and gene expression analysis to uncover resistance in the Yugu1 cultivar, which is known for its innate immunity. Our analysis identified three genomic regions associated with resistance, and fine mapping pinpointed a critical quantitative trait locus, <i>qLB-03.1</i>, within a 56.8 kb interval on chromosome 3. Eight genes were annotated in this region, and time series expression analysis revealed that <i>Seita.3G119300</i>, which encodes a cysteine-rich secretory protein, was significantly upregulated following inoculation. This upregulation suggests a crucial role in the resistance phenotype. Intriguingly, a significant 4592 bp deletion in the promoter region of <i>Seita.3G119300</i> in the susceptible Ci846 cultivar appears to affect its expression and susceptibility to the disease. This deletion highlights <i>Seita.3G119300</i> (tentatively named <i>Rpg3(t)</i>) as a candidate resistance gene, warranting functional validation and underscoring its potential for inclusion in breeding programs aimed at enhancing blast resistance in foxtail millet. Continued research is needed to fully validate the functional role of this gene and harness its potential for enhancing crop resilience.</p>

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Identification and fine mapping of blast resistance gene Rpg3(t) in foxtail millet (Setaria italica L.)

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年10月21日

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  • Xinge Xia,
  • Muhammad Sohaib Shafique,
  • Weiqing Huang,
  • Yapei Liu,
  • Hui Zhi,
  • Chunlian Wang,
  • Yi Zhou,
  • Zhiyuan Ji

摘要

Foxtail millet (Setaria italica L.) is an important cereal crop, resilient to abiotic stresses, but suffers significant yield losses from blast disease, caused by Pyricularia grisea. Understanding the genetic mechanisms underlying blast resistance is essential for developing resistant cultivars. This study employed bulk segregant analysis sequencing and gene expression analysis to uncover resistance in the Yugu1 cultivar, which is known for its innate immunity. Our analysis identified three genomic regions associated with resistance, and fine mapping pinpointed a critical quantitative trait locus, qLB-03.1, within a 56.8 kb interval on chromosome 3. Eight genes were annotated in this region, and time series expression analysis revealed that Seita.3G119300, which encodes a cysteine-rich secretory protein, was significantly upregulated following inoculation. This upregulation suggests a crucial role in the resistance phenotype. Intriguingly, a significant 4592 bp deletion in the promoter region of Seita.3G119300 in the susceptible Ci846 cultivar appears to affect its expression and susceptibility to the disease. This deletion highlights Seita.3G119300 (tentatively named Rpg3(t)) as a candidate resistance gene, warranting functional validation and underscoring its potential for inclusion in breeding programs aimed at enhancing blast resistance in foxtail millet. Continued research is needed to fully validate the functional role of this gene and harness its potential for enhancing crop resilience.