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<p><i>Meloidogyne hispanica</i> has received significant attention as it poses a major threat to various crops due to its expanding geographical distribution. Consequently, there is an urgent need for specific diagnostic techniques targeting <i>M. hispanica</i>. However, the development of effective molecular diagnostic methods has been hindered by the lack of genomic information. To address this challenge, we developed a species-specific SCAR-PCR assay based on a RAPD marker of <i>M. hispanica</i> in this study. The SCAR primers were designed from the RAPD amplicon, yielding a 385-bp fragment exclusively amplified from the genome of <i>M. hispanica</i>. This method successfully amplified DNA from <i>M. hispanica</i> at all developmental stages and from infected soil samples. It exhibited high sensitivity, with detection limits equivalent to 1/1280 of a single female and 1/160 of a second-stage juvenile. This method provides a reliable tool for the specific detection of <i>M. hispanica</i>, enabling timely monitoring and effective containment of its spread.</p>

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Molecular diagnosis of the Seville root-knot nematode Meloidogyne hispanica based on SCAR marker

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年10月28日

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  • Yuan Chen,
  • Li Wang,
  • Yanfang Sun,
  • Tuizi Feng,
  • Yueling Pei,
  • Deliang Peng,
  • Haibo Long

摘要

Meloidogyne hispanica has received significant attention as it poses a major threat to various crops due to its expanding geographical distribution. Consequently, there is an urgent need for specific diagnostic techniques targeting M. hispanica. However, the development of effective molecular diagnostic methods has been hindered by the lack of genomic information. To address this challenge, we developed a species-specific SCAR-PCR assay based on a RAPD marker of M. hispanica in this study. The SCAR primers were designed from the RAPD amplicon, yielding a 385-bp fragment exclusively amplified from the genome of M. hispanica. This method successfully amplified DNA from M. hispanica at all developmental stages and from infected soil samples. It exhibited high sensitivity, with detection limits equivalent to 1/1280 of a single female and 1/160 of a second-stage juvenile. This method provides a reliable tool for the specific detection of M. hispanica, enabling timely monitoring and effective containment of its spread.