Scholarlink

Background <p>Verticillium wilt, commonly known as cotton cancer, is a devastating disease that significantly reduces cotton yield and fiber quality worldwide. The nuclear pore complex (NPC), composed of nucleoporins (Nups), plays a crucial role in plant disease resistance by&#xa0;mediating nucleocytoplasmic transport and selective nuclear signaling that are key components of pathogen defense. Given the critical role of nucleoporins in plant immunity, their specific roles in cotton remain poorly understood, highlighting the need for a comprehensive bioinformatics analysis to clarify their contributions to disease resistance in cotton.</p> Results <p>This study identified a total of 42 <i>Nup</i> genes across five cotton species. Phylogenetic analysis revealed divergence of these genes into six distinct subgroups during evolution. Analysis of <i>cis</i>-acting elements indicated that <i>Nup</i> gene expression may be regulated by salicylic acid (SA), jasmonic acid (JA), and temperature, impacting disease resistance. Collinearity and chromosomal mapping demonstrated strong conservation of <i>Nup</i> gene functions across species. Furthermore, virus-induced gene silencing (VIGS) and quantitative real-time polymerase chain reaction (RT-qPCR) showed that silencing of <i>Ghnup160</i> significantly reduced cotton resistance to Verticillium wilt.</p> Conclusions <p>Our study suggests an important role of <i>Ghnup160</i> in the cotton defense pathway and provides functional insights into the <i>Ghnup160</i> gene and lays the groundwork for further investigation into Nup-mediated resistance mechanisms in cotton.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

GhNUP160 mediates cotton resistance to Verticillium dahliae through salicylic acid and jasmonic acid pathways

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年4月9日

查看全文
  • Zhang Guangxing,
  • Wang Heng,
  • Zheng Jie,
  • Liu Yiman,
  • Zhang Menghan,
  • Liu Zhuang,
  • Zhang Yuanyuan,
  • Cai Xiaoyan,
  • Hou Yuqing,
  • Zhou Zhongli,
  • Liu Fang,
  • Li Zhikun,
  • Xu Yanchao

摘要

Background

Verticillium wilt, commonly known as cotton cancer, is a devastating disease that significantly reduces cotton yield and fiber quality worldwide. The nuclear pore complex (NPC), composed of nucleoporins (Nups), plays a crucial role in plant disease resistance by mediating nucleocytoplasmic transport and selective nuclear signaling that are key components of pathogen defense. Given the critical role of nucleoporins in plant immunity, their specific roles in cotton remain poorly understood, highlighting the need for a comprehensive bioinformatics analysis to clarify their contributions to disease resistance in cotton.

Results

This study identified a total of 42 Nup genes across five cotton species. Phylogenetic analysis revealed divergence of these genes into six distinct subgroups during evolution. Analysis of cis-acting elements indicated that Nup gene expression may be regulated by salicylic acid (SA), jasmonic acid (JA), and temperature, impacting disease resistance. Collinearity and chromosomal mapping demonstrated strong conservation of Nup gene functions across species. Furthermore, virus-induced gene silencing (VIGS) and quantitative real-time polymerase chain reaction (RT-qPCR) showed that silencing of Ghnup160 significantly reduced cotton resistance to Verticillium wilt.

Conclusions

Our study suggests an important role of Ghnup160 in the cotton defense pathway and provides functional insights into the Ghnup160 gene and lays the groundwork for further investigation into Nup-mediated resistance mechanisms in cotton.