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Background <p>Widespread in many tropical and subtropical regions of the world, dengue fever is currently the most serious public health problem posed by arboviruses. Data on this disease are scarce due to the similarity of its symptoms to those of malaria, which is endemic in this region. This study aimed to evaluate the epidemiological, clinical, and paraclinical aspects of dengue fever in the city of Kribi, thereby providing objective data that could characterize the circulation of this disease in this city.</p> Methods <p>We conducted a cross-sectional descriptive and analytical study from November 2024 to June 2025 at the Kribi Regional Annex Hospital. We included febrile patients to whom venous blood samples were collected in EDTA tubes. The presence of IgM, NS1, and IgG anti-DENV was detected using the lateral flow immunoassay technique. Viral genome testing was performed on samples positive for Ig Met NS1 using the TrioPlex RT-qPCR DENV, ZIKV, CHICKV‘protocol, after first extracting viral RNA using the ’Daan Gene RNA/DNA purification” kit. The data obtained were analyzed using SPSS 25.</p> Results <p>Of the 277 patients recruited, 8.7% (24/277) were positive for IgM, 3.6% (10/277) for NS1 Ag, and 41.5% (115/277) for IgG. The frequency of dengue (IgM/NS1 positive) was 10.5% (<i>n</i> = 29) with 3.5% (1/29) positive by RT-qPCR. More than half of the dengue cases (IgM/NS1) were female 17(58.6%). In addition to fever, the main clinical signs were headaches (72.4%), abdominal pain (58.6%), and dizziness (55.2%). Proximity to the forest increased the risk of contracting dengue (OR = 2.33). The concordance between tests was moderate between the NS1 and IgM tests, low between the RT-qPCR and NS1 tests on the one hand and between the RT-qPCR and IgM tests on the other.</p> Conclusion <p>The dengue virus is present in the city of Kribi and was found in 10.5% of individuals with fever. A high frequency of IgG (115/277) has been observed, indicating silent circulation in the community and spontaneous recoveries.</p>

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Epidemiological, clinical and paraclinical aspects of dengue fever in the city of Kribi, Southern Cameroon

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年7月3日

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  • Makemgue Louise Stéphanie,
  • Bidzang Jean Arsène,
  • Atsama Amougou Marie,
  • Tankoua-Tchounda Roméo,
  • Nguemaim Ngoufo Flore,
  • Nemg Simo Fredy Brice,
  • Simo Stéphanie,
  • Nfegue Marie Thérèse,
  • Bengono Bengono Roddy Stéphan

摘要

Background

Widespread in many tropical and subtropical regions of the world, dengue fever is currently the most serious public health problem posed by arboviruses. Data on this disease are scarce due to the similarity of its symptoms to those of malaria, which is endemic in this region. This study aimed to evaluate the epidemiological, clinical, and paraclinical aspects of dengue fever in the city of Kribi, thereby providing objective data that could characterize the circulation of this disease in this city.

Methods

We conducted a cross-sectional descriptive and analytical study from November 2024 to June 2025 at the Kribi Regional Annex Hospital. We included febrile patients to whom venous blood samples were collected in EDTA tubes. The presence of IgM, NS1, and IgG anti-DENV was detected using the lateral flow immunoassay technique. Viral genome testing was performed on samples positive for Ig Met NS1 using the TrioPlex RT-qPCR DENV, ZIKV, CHICKV‘protocol, after first extracting viral RNA using the ’Daan Gene RNA/DNA purification” kit. The data obtained were analyzed using SPSS 25.

Results

Of the 277 patients recruited, 8.7% (24/277) were positive for IgM, 3.6% (10/277) for NS1 Ag, and 41.5% (115/277) for IgG. The frequency of dengue (IgM/NS1 positive) was 10.5% (n = 29) with 3.5% (1/29) positive by RT-qPCR. More than half of the dengue cases (IgM/NS1) were female 17(58.6%). In addition to fever, the main clinical signs were headaches (72.4%), abdominal pain (58.6%), and dizziness (55.2%). Proximity to the forest increased the risk of contracting dengue (OR = 2.33). The concordance between tests was moderate between the NS1 and IgM tests, low between the RT-qPCR and NS1 tests on the one hand and between the RT-qPCR and IgM tests on the other.

Conclusion

The dengue virus is present in the city of Kribi and was found in 10.5% of individuals with fever. A high frequency of IgG (115/277) has been observed, indicating silent circulation in the community and spontaneous recoveries.