<p>Stem rust caused by <i>Puccinia graminis</i> f. sp. <i>tritici</i> continues to pose a serious risk to wheat cultivation because newly emerging pathogen races frequently overcome existing resistance. Traditional wheat landraces represent valuable genetic resources that can contribute novel resistance alleles for crop improvement. In the present investigation, Pakistani wheat landraces obtained from the USDA National Small Grains Collection were evaluated using SSR markers linked with the resistance genes <i>Sr</i>22 and <i>Sr</i>24. The markers CFA2019 and CFA2123 were applied for <i>Sr</i>22 detection, whereas BARC71 was used for <i>Sr</i>24. The screening results revealed that <i>Sr</i>22-associated alleles were present in a large proportion of accessions, while <i>Sr</i>24 occurred in relatively few genotypes. These outcomes highlight the breeding potential of Pakistani landraces and support the utilization of <i>Sr</i>22-carrying materials in marker-assisted breeding programs aimed at strengthening stem rust resistance in wheat.</p>

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SSR marker-based detection of stem rust resistance genes (Sr22 and Sr24) in Pakistani wheat landraces

  • Muhammad Siddique Samoo,
  • Hadi Bux

摘要

Stem rust caused by Puccinia graminis f. sp. tritici continues to pose a serious risk to wheat cultivation because newly emerging pathogen races frequently overcome existing resistance. Traditional wheat landraces represent valuable genetic resources that can contribute novel resistance alleles for crop improvement. In the present investigation, Pakistani wheat landraces obtained from the USDA National Small Grains Collection were evaluated using SSR markers linked with the resistance genes Sr22 and Sr24. The markers CFA2019 and CFA2123 were applied for Sr22 detection, whereas BARC71 was used for Sr24. The screening results revealed that Sr22-associated alleles were present in a large proportion of accessions, while Sr24 occurred in relatively few genotypes. These outcomes highlight the breeding potential of Pakistani landraces and support the utilization of Sr22-carrying materials in marker-assisted breeding programs aimed at strengthening stem rust resistance in wheat.