Background <p>Uncontrolled bleeding during partial nephrectomy poses a significant surgical challenge. This study evaluates the hemostatic efficacy and acute histopathological effects of Algan Hemostatic Agent (AHA), a novel plant-based agent, in a rat model of partial nephrectomy.</p> Methods <p>Twenty-four rats underwent partial nephrectomy and were randomized to three groups: control (saline), AHA powder, and AHA liquid-impregnated sponge. Hemostasis was attempted for up to 3 applications, and success rates were compared. Kidney tissue was harvested for histopathological evaluation, assessing tissue necrosis, coagulation, hemorrhage, and inflammation.</p> Results <p>AHA powder achieved hemostasis in all 8 rats (100%) after a single application, which was significantly higher than the control group (<i>p</i> &lt; 0.001). The AHA liquid-impregnated sponge achieved hemostasis in 6 out of 8 rats (75%) after the first application and in all 8 rats (100%) after the second. No significant histopathological damage was observed in any group.</p> Conclusion <p>AHA, particularly in powder form, demonstrates promising hemostatic efficacy during partial nephrectomy in rats with no significant acute histopathological damage. Further research is warranted to evaluate long-term effects and compare AHA with existing hemostatic agents</p>