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Background <p>Endometriosis is a benign gynecological disease characterized by the growth of endometrial cells beyond the uterus, forming endometriotic cyst tissues called ovarian endometriomas. MicroRNAs (miRNAs) are small, non-coding RNAs that epigenetically control the physiological and pathological processes of different diseases, including endometriosis. In this study, we screened the expression levels of 11-selected miRNAs, namely miR-21-5p, miR-200c-3p, miR-19a-3p, miR-203-3p, miR-181b-5p, miR-182-5p, miR-let7a-5p, miR-205-5p, miR-200b-3p, miR-16-5p, and miR-222-3p in ovarian endometriomas relative to eutopic endometrial tissues using quantitative real-time PCR (qPCR). In addition, the level of mRNA expression of lumican (<i>LUM</i>), an extracellular matrix proteoglycan (PG), and a putative target of miR-21-5p was quantified by qPCR.</p> Results <p>Our screening qPCR results showed that 9 miRNAs were upregulated (miR-21-5p, miR-200c-3p, miR-19a-3p, miR-203-3p, miR-181b-5p, miR-182-5p, miR-let7a-5p, miR-205-5p, and miR-200b-3p), whereas 2 miRNAs were downregulated (miR-16-5p and miR-222-3p) in ovarian endometriomas compared to eutopic endometrium. A significant overexpression of miR-21-5p in ovarian endometrioma was further independently verified by qPCR. Using bioinformatics tools, Gene Ontology Kyoto Encyclopedia of Genes and Genomes pathways, and protein–protein interactions, we identified differentially expressed genes and several pathways regulated by miR-21-5p that may contribute to endometriosis progression. Among them, <i>LUM</i> was found to be significantly diminished expressed in ovarian endometriomas compared to eutopic endometrium.</p> Conclusion <p>In conclusion, this study identified miR-21-5p and <i>LUM</i> as potential factors that may contribute to ovarian endometriomas’ pathogenesis.</p>

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Overexpression of miR-21-5p as a potential pathogenesis marker for ovarian endometriosis

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年1月4日

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  • Mohamed Mahmoud,
  • Amr Ahmed WalyEldeen,
  • Mohamed A. Samie,
  • Ahmed M. Sadek,
  • Sayed Elakhras,
  • Mohamed Samir,
  • Osama Ahmed,
  • Rasha Mohamed Samir Sayed,
  • Ahmed Abdelaziz Baiomy,
  • Sherif Abdelaziz Ibrahim,
  • Hebatallah Hassan

摘要

Background

Endometriosis is a benign gynecological disease characterized by the growth of endometrial cells beyond the uterus, forming endometriotic cyst tissues called ovarian endometriomas. MicroRNAs (miRNAs) are small, non-coding RNAs that epigenetically control the physiological and pathological processes of different diseases, including endometriosis. In this study, we screened the expression levels of 11-selected miRNAs, namely miR-21-5p, miR-200c-3p, miR-19a-3p, miR-203-3p, miR-181b-5p, miR-182-5p, miR-let7a-5p, miR-205-5p, miR-200b-3p, miR-16-5p, and miR-222-3p in ovarian endometriomas relative to eutopic endometrial tissues using quantitative real-time PCR (qPCR). In addition, the level of mRNA expression of lumican (LUM), an extracellular matrix proteoglycan (PG), and a putative target of miR-21-5p was quantified by qPCR.

Results

Our screening qPCR results showed that 9 miRNAs were upregulated (miR-21-5p, miR-200c-3p, miR-19a-3p, miR-203-3p, miR-181b-5p, miR-182-5p, miR-let7a-5p, miR-205-5p, and miR-200b-3p), whereas 2 miRNAs were downregulated (miR-16-5p and miR-222-3p) in ovarian endometriomas compared to eutopic endometrium. A significant overexpression of miR-21-5p in ovarian endometrioma was further independently verified by qPCR. Using bioinformatics tools, Gene Ontology Kyoto Encyclopedia of Genes and Genomes pathways, and protein–protein interactions, we identified differentially expressed genes and several pathways regulated by miR-21-5p that may contribute to endometriosis progression. Among them, LUM was found to be significantly diminished expressed in ovarian endometriomas compared to eutopic endometrium.

Conclusion

In conclusion, this study identified miR-21-5p and LUM as potential factors that may contribute to ovarian endometriomas’ pathogenesis.