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<p>Fruit and vegetable processing waste is a considerable underestimated source of bioactive phytochemicals that can be useful for nutrition/reproductive health. However, currently available literature does not provide any critical integration of analytical methodologies in relation to bioactivity and bioavailability and translational relevance. This review gives a systematic review of analytical approaches employed for phytochemical profiling of food co-products covering chromatographic, spectrometric, and metabolomics approaches. A structured literature search was undertaken in Scopus, PubMed and Web of Science database (2015–2025). Comparative evaluation suggests that while conventional techniques like the most common methods like the High-Pressure Liquid Chromatography (HPLC) are suitable for routine quantification purposes, advanced techniques commercially used like the LC-Mass Spectrometry (LC-MS/MS) and Metabolomics provide a superior structural resolution and a comprehensive profiling. Importantly, the review makes critical connections between analytical data and biological relevance, thus highlighting the limitations attributed to dose, bioavailability and to the clinical translation. This work presents a novel integrative approach linking analytical chemistry to nutritional outcomes and sustainable waste valorization of food.</p> Graphical Abstract <p></p>

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Bioactive phytochemicals in fruit and vegetable waste: analytical strategies for chemical profiling and health-oriented applications

  • 期刊论文

  • 发表日期：

    2026年5月16日

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  • Mahesh Sonkamble,
  • Shoaeb Mohammad Syed

摘要

Fruit and vegetable processing waste is a considerable underestimated source of bioactive phytochemicals that can be useful for nutrition/reproductive health. However, currently available literature does not provide any critical integration of analytical methodologies in relation to bioactivity and bioavailability and translational relevance. This review gives a systematic review of analytical approaches employed for phytochemical profiling of food co-products covering chromatographic, spectrometric, and metabolomics approaches. A structured literature search was undertaken in Scopus, PubMed and Web of Science database (2015–2025). Comparative evaluation suggests that while conventional techniques like the most common methods like the High-Pressure Liquid Chromatography (HPLC) are suitable for routine quantification purposes, advanced techniques commercially used like the LC-Mass Spectrometry (LC-MS/MS) and Metabolomics provide a superior structural resolution and a comprehensive profiling. Importantly, the review makes critical connections between analytical data and biological relevance, thus highlighting the limitations attributed to dose, bioavailability and to the clinical translation. This work presents a novel integrative approach linking analytical chemistry to nutritional outcomes and sustainable waste valorization of food.

Graphical Abstract