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<p>Adipose tissue dysfunction and adipogenesis are the pivotal events that are intricately linked to lipid disorders, notably encompassing hyperlipidemia and obesity. Here, we investigated transcriptome alterations in the visceral adipose tissue of male Wistar albino rats fed a high-fat diet, following the treatment with proso millet malt extract, through RNA sequencing technology. <i>In-vivo</i> anti-hyperlipidemic studies showed increased weight gain in high fat diet fed animals (130.67 ± 22.7&#xa0;g), whereas proso-millet fed animals showed subsequent decrease in body weight (67.67 ± 15.2&#xa0;g). The millet treatment also demonstrated significant improvement in lipid profile and liver function. Histopathological studies of adipose tissue revealed hypertrophy and hyperplasia conditions elevated in high fat fed animals was mitigated in animals fed with proso millet malt. Comprehensive transcriptomic profiling of HFD adipose tissues projected 8030 differentially expressed genes out of which 859 genes were significantly down-regulated in the adipose tissue transcriptome of proso millet-treated rats. Gene ontology analysis showed the involvement of genes in some of the prominent adipogenesis and adipocyte dysfunction-associated signaling pathways. Most of the genes involved in the PI3/AKT signaling pathway were found to be significantly down-regulated in proso millet-treated rats. Further disease pathway analysis showed <i>Fos</i> and <i>Mapk</i> genes to be the bottleneck proteins that play a major role in <i>the Il-6</i> signaling pathway. Our findings provide compelling evidence that proso millet malt has significant potential for managing lipid disorders. This is achieved through its modulation of <i>Fos</i> and <i>MAPK</i> proteins within the PI3/AKT signaling pathway, which is crucial for adipogenesis. This suggests a novel avenue for therapeutic intervention in lipid disorders. We present one of the first studies focusing on the alteration of gene expression profiles in visceral adipose tissue induced by proso millet malt.</p>

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Transcriptome profiling uncovered the therapeutic potentials of proso millet malt in regulating hyperlipidemia

  • 期刊论文

  • 发表日期：

    2025年7月9日

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  • S. Nivedita Pujari,
  • H. Joy Hoskeri

摘要

Adipose tissue dysfunction and adipogenesis are the pivotal events that are intricately linked to lipid disorders, notably encompassing hyperlipidemia and obesity. Here, we investigated transcriptome alterations in the visceral adipose tissue of male Wistar albino rats fed a high-fat diet, following the treatment with proso millet malt extract, through RNA sequencing technology. In-vivo anti-hyperlipidemic studies showed increased weight gain in high fat diet fed animals (130.67 ± 22.7 g), whereas proso-millet fed animals showed subsequent decrease in body weight (67.67 ± 15.2 g). The millet treatment also demonstrated significant improvement in lipid profile and liver function. Histopathological studies of adipose tissue revealed hypertrophy and hyperplasia conditions elevated in high fat fed animals was mitigated in animals fed with proso millet malt. Comprehensive transcriptomic profiling of HFD adipose tissues projected 8030 differentially expressed genes out of which 859 genes were significantly down-regulated in the adipose tissue transcriptome of proso millet-treated rats. Gene ontology analysis showed the involvement of genes in some of the prominent adipogenesis and adipocyte dysfunction-associated signaling pathways. Most of the genes involved in the PI3/AKT signaling pathway were found to be significantly down-regulated in proso millet-treated rats. Further disease pathway analysis showed Fos and Mapk genes to be the bottleneck proteins that play a major role in the Il-6 signaling pathway. Our findings provide compelling evidence that proso millet malt has significant potential for managing lipid disorders. This is achieved through its modulation of Fos and MAPK proteins within the PI3/AKT signaling pathway, which is crucial for adipogenesis. This suggests a novel avenue for therapeutic intervention in lipid disorders. We present one of the first studies focusing on the alteration of gene expression profiles in visceral adipose tissue induced by proso millet malt.