Purpose <p>To determine the gastroprotective effect of the ethanol extract of <i>Ficus virens</i> Aiton bark (EFVAB) against ethanol-induced gastric ulcers in rats.</p> Methods <p>Dried powdered bark of <i>Ficus virens</i> was extracted by Soxhlet extraction using ethanol as a solvent, yielding 12.05%, and preliminary phytochemical screening of the ethanolic bark extract was performed. Male Wistar rats were divided into five groups of six animals each. Groups I and II received normal saline as vehicle, groups III to IV received 200 and 400 mg/kg of extract, and group V received 20 mg/kg omeprazole as the standard drug, respectively, for 7 days. Gastric ulceration was induced on the 7th day after 2 h of drug administration by administering 96% ethanol (5 ml/kg, b.w.) to groups II, III, IV, and V. Animals were euthanized by intraperitoneal administration of ketamine (80 mg/kg) and xylazine (10 mg/kg), 1 h after ethanol administration. The stomachs were isolated and various parameters including ulcer index, gastric pH, free acidity, and total acidity were determined. Molecular docking of the reported phytoconstituents of <i>Ficus virens</i> against acid-secreting enzyme H⁺/K⁺ ATPase enzyme was carried out using PDP: 2XZB.</p> Results <p>Extract at highest dose (400 mg/kg, p.o.) and omeprazole were more effective in reducing ulcer index after 7 days treatment period. In addition, EFVAB, at both doses, increased gastric pH significantly, reduced free acidity and total acidity significantly when compared with diseased group. Docking study also confirmed gastroprotective effect of bark by showing good binding affinity towards H⁺/K⁺ ATPase enzyme site.</p> Conclusion <p>Our findings suggest that ethanol extract of <i>Ficus virens</i> Aiton bark is gastroprotective against ethanol-induced gastric ulcer possibly through increased gastric pH, reduction in gastric acid secretion, and decreased free and total acidity, thus leading to decrease in ulcer index after ethanol exposure.</p>