Scholarlink

Objectives <p>This study evaluated the protective effects of geraniol (GER) on hepatic ischemia/reperfusion (I/R) injury in rats by assessing cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) levels.</p> Methods <p>Rats were divided into seven groups: sham, I/R with 1 or 6&#xa0;h of reperfusion, and I/R treated with GER (50 or 100&#xa0;mg/kg) before 1 or 6&#xa0;h of reperfusion. Serum liver enzymes were measured to assess liver function, while mRNA and protein levels of IL-6, IL-10, COX-2, and iNOS were analyzed using qRT-PCR and ELISA. Statistical analysis was performed using one-way ANOVA followed by Tukey’s post hoc test.</p> Results <p>GER treatment significantly reduced serum aminotransferase levels, histological damage, and inflammatory markers. In the I/R group, COX-2 and iNOS levels were significantly elevated compared to the sham group. GER administration markedly decreased IL-6, IL-10, COX-2, and iNOS levels compared to the I/R group. Histopathological analysis revealed increased vascularization and necrosis in the untreated I/R group, which were attenuated by GER at both doses.</p> Conclusions <p>These findings suggest that GER’s anti-inflammatory properties contribute to its hepatoprotective effects in I/R injury by reducing COX-2 and iNOS expression. GER shows promise as a therapeutic agent for managing hepatic I/R injury, warranting further clinical investigation.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Effect of geraniol on cyclooxygenase-2 and inducible nitric oxide synthase levels in a rat hepatic ischemia/reperfusion model

  • 期刊论文

  • 发表日期：

    2025年3月31日

查看全文
  • Sara Shafieipour,
  • Hamid Khajehpour,
  • Shahriyar Dabiri,
  • Mohsen Nakhaei,
  • Seyedeh Mahdieh Khoshnazar

摘要

Objectives

This study evaluated the protective effects of geraniol (GER) on hepatic ischemia/reperfusion (I/R) injury in rats by assessing cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) levels.

Methods

Rats were divided into seven groups: sham, I/R with 1 or 6 h of reperfusion, and I/R treated with GER (50 or 100 mg/kg) before 1 or 6 h of reperfusion. Serum liver enzymes were measured to assess liver function, while mRNA and protein levels of IL-6, IL-10, COX-2, and iNOS were analyzed using qRT-PCR and ELISA. Statistical analysis was performed using one-way ANOVA followed by Tukey’s post hoc test.

Results

GER treatment significantly reduced serum aminotransferase levels, histological damage, and inflammatory markers. In the I/R group, COX-2 and iNOS levels were significantly elevated compared to the sham group. GER administration markedly decreased IL-6, IL-10, COX-2, and iNOS levels compared to the I/R group. Histopathological analysis revealed increased vascularization and necrosis in the untreated I/R group, which were attenuated by GER at both doses.

Conclusions

These findings suggest that GER’s anti-inflammatory properties contribute to its hepatoprotective effects in I/R injury by reducing COX-2 and iNOS expression. GER shows promise as a therapeutic agent for managing hepatic I/R injury, warranting further clinical investigation.