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Objective <p>This study aimed to explore the biological function of miR-1229-3p in the occurrence and development of cervical cancer (CC).</p> Methods <p>The expression levels of miR-1229-3p and FBXL5 in CC tissues and cell lines were detected by RT-qPCR. Cell proliferation ability was evaluated by MTT. Cell migration and invasion abilities were detected by Transwell. The expression of EMT-related markers were detected by RT-qPCR. The targeting relationship between miR-1229-3p and FBXL5 was verified by dual luciferase reporter assay.</p> Results <p>The expression of miR-1229-3p was significantly upregulated in CC tissues and CC cell lines. High expression of miR-1229-3p was associated with poor differentiation and lymph node metastasis. Overexpression of miR-1229-3p could promote the proliferation, migration, invasion of CC cells, and induce the EMT process. Dual luciferase reporter assays confirmed that FBXL5 was the direct target gene of miR-1229-3p, and their expressions showed a significant negative correlation in CC tissues. Overexpression of FBXL5 could reverse the carcinogenic effects caused by miR-1229-3p.</p> Conclusion <p>miR-1229-3p directly inhibits the expression of FBXL5, thereby activating the EMT process, and ultimately promoting the proliferation, migration, and invasion of CC cells.</p>

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miR-1229-3p promotes epithelial-mesenchymal transition and metastasis of cervical cancer cells by targeting FBXL5

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年4月22日

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  • Donglian Lan,
  • Xiaojing Sun,
  • Lei Yao,
  • Bo Cao

摘要

Objective

This study aimed to explore the biological function of miR-1229-3p in the occurrence and development of cervical cancer (CC).

Methods

The expression levels of miR-1229-3p and FBXL5 in CC tissues and cell lines were detected by RT-qPCR. Cell proliferation ability was evaluated by MTT. Cell migration and invasion abilities were detected by Transwell. The expression of EMT-related markers were detected by RT-qPCR. The targeting relationship between miR-1229-3p and FBXL5 was verified by dual luciferase reporter assay.

Results

The expression of miR-1229-3p was significantly upregulated in CC tissues and CC cell lines. High expression of miR-1229-3p was associated with poor differentiation and lymph node metastasis. Overexpression of miR-1229-3p could promote the proliferation, migration, invasion of CC cells, and induce the EMT process. Dual luciferase reporter assays confirmed that FBXL5 was the direct target gene of miR-1229-3p, and their expressions showed a significant negative correlation in CC tissues. Overexpression of FBXL5 could reverse the carcinogenic effects caused by miR-1229-3p.

Conclusion

miR-1229-3p directly inhibits the expression of FBXL5, thereby activating the EMT process, and ultimately promoting the proliferation, migration, and invasion of CC cells.