Scholarlink

Background <p>In terms of global incidence and mortality, breast cancer continues to surpass all other cancers affecting women.</p> Methods <p>To explore the role of miR-596, qRT-PCR was applied to measure its expression in tissue and cell samples from 137 enrolled subjects. The regulatory interaction between miR-596 and <i>EIF5AL1</i> was verified by dual-luciferase reporter assays. CCK-8 and Transwell assays were utilized to respectively measure the proliferation, migration, and invasion capabilities of the two breast cancer cell lines, MCF-7 and MDA-MB-231.</p> Results <p>A significant downregulation of miR-596 was observed in breast cancer tissues and cell lines (all <i>P</i> &lt; 0.001). Clinically, reduced miR-596 expression was associated with advanced TNM stage, lymph node metastasis, and inferior overall survival (<i>P</i> &lt; 0.05). <i>EIF5AL1</i> was validated as a direct target gene of miR-596, and their expression levels were inversely correlated in clinical samples (<i>r</i> = -0.801, <i>P</i> &lt; 0.001). Reintroduction of miR-596 markedly suppressed the proliferation, migration, and invasion of cancer cells, effects that were largely reversed by overexpressing <i>EIF5AL1</i> (all <i>P</i> &lt; 0.001).</p> Conclusion <p>In breast cancer, miR-596 suppresses malignancy and predicts prognosis by targeting <i>EIF5AL1</i>. Thus, therapeutic modulation of this axis offers novel avenues for treatment and risk assessment.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

miR-596 as a novel prognostic biomarker and tumor suppressor in breast cancer through targeting EIF5AL1

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年1月22日

查看全文
  • Rui Huang,
  • Yan Jiang,
  • Yan Bian,
  • Dengyuan Zhong,
  • Baoyong Lv

摘要

Background

In terms of global incidence and mortality, breast cancer continues to surpass all other cancers affecting women.

Methods

To explore the role of miR-596, qRT-PCR was applied to measure its expression in tissue and cell samples from 137 enrolled subjects. The regulatory interaction between miR-596 and EIF5AL1 was verified by dual-luciferase reporter assays. CCK-8 and Transwell assays were utilized to respectively measure the proliferation, migration, and invasion capabilities of the two breast cancer cell lines, MCF-7 and MDA-MB-231.

Results

A significant downregulation of miR-596 was observed in breast cancer tissues and cell lines (all P < 0.001). Clinically, reduced miR-596 expression was associated with advanced TNM stage, lymph node metastasis, and inferior overall survival (P < 0.05). EIF5AL1 was validated as a direct target gene of miR-596, and their expression levels were inversely correlated in clinical samples (r = -0.801, P < 0.001). Reintroduction of miR-596 markedly suppressed the proliferation, migration, and invasion of cancer cells, effects that were largely reversed by overexpressing EIF5AL1 (all P < 0.001).

Conclusion

In breast cancer, miR-596 suppresses malignancy and predicts prognosis by targeting EIF5AL1. Thus, therapeutic modulation of this axis offers novel avenues for treatment and risk assessment.