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Purpose <p>This study aims to investigate the distinct behaviors of single-species and dual-species biofilms formed by <i>Streptococcus sanguinis</i> and <i>Aggregatibacter actinomycetemcomitans</i> on different titanium and implant surfaces. Four types of surfaces were examined: two clinically used implant surfaces, a super-polished surface and a sand-blasted surface of grade 4 titanium.</p> Methods <p>Specimens were incubated with single- and dual-species biofilms for 24&#xa0;h. Biofilm formation was determined based on the amount of total DNA extracted from the bacteria. In order to specifically determine the biofilm formation of <i>Streptococcus sanguinis</i>, qPCR experiments were carried out. Staining followed by fluorescence microscopy was employed to verify the efficiency of the washing steps.</p> Results <p>Biofilm formation by single- and dual-species cultures was observed on all tested implant surfaces. However, a clear influence of surface characteristics on biofilm formation could not be conclusively demonstrated. Mixed cultures of <i>S. sanguinis</i> and <i>A. actinomycetemcomitans</i> (<i>AAC</i>) exhibited increased biofilm formation through the enhanced DNA amount of <i>S. sanguinis</i>. In contrast, this effect was not observed in dual-species cultures of <i>Staphylococcus epidermidis</i> and <i>S. sanguinis</i>.</p> Conclusion <p><i>AAC</i> promotes biofilm formation of <i>S. sanguinis</i>, highlighting the significant role of <i>AAC</i> in enhancing biofilm development. Conversely, a definitive conclusion regarding the correlation between titanium implant surface roughness and biofilm formation was not possible. However, our results suggest a tendency that dual-species biofilm formation may be influenced by surface structure.</p>

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Aggregatibacter actinomycetemcomitans induces biofilm formation of Streptococcus sanguinis on titanium implants

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年4月3日

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  • Andrea Schubert,
  • Jana Marisa Friebel,
  • Oskar Bunz,
  • Christoph Sasse,
  • Ralf Bürgers,
  • Torsten Wassmann

摘要

Purpose

This study aims to investigate the distinct behaviors of single-species and dual-species biofilms formed by Streptococcus sanguinis and Aggregatibacter actinomycetemcomitans on different titanium and implant surfaces. Four types of surfaces were examined: two clinically used implant surfaces, a super-polished surface and a sand-blasted surface of grade 4 titanium.

Methods

Specimens were incubated with single- and dual-species biofilms for 24 h. Biofilm formation was determined based on the amount of total DNA extracted from the bacteria. In order to specifically determine the biofilm formation of Streptococcus sanguinis, qPCR experiments were carried out. Staining followed by fluorescence microscopy was employed to verify the efficiency of the washing steps.

Results

Biofilm formation by single- and dual-species cultures was observed on all tested implant surfaces. However, a clear influence of surface characteristics on biofilm formation could not be conclusively demonstrated. Mixed cultures of S. sanguinis and A. actinomycetemcomitans (AAC) exhibited increased biofilm formation through the enhanced DNA amount of S. sanguinis. In contrast, this effect was not observed in dual-species cultures of Staphylococcus epidermidis and S. sanguinis.

Conclusion

AAC promotes biofilm formation of S. sanguinis, highlighting the significant role of AAC in enhancing biofilm development. Conversely, a definitive conclusion regarding the correlation between titanium implant surface roughness and biofilm formation was not possible. However, our results suggest a tendency that dual-species biofilm formation may be influenced by surface structure.