<p>Ti6Al4V and 316L stainless steel alloys have been extensively utilized as conventional implant materials. However, there are always concerns regarding the biocompatibility of these alloys. Recently, high-entropy alloys have been introduced as new biomaterials due to their unique properties. In this investigation, a Ti₃₅Nb₂₅Zr₁₅Mo₁₅V₁₀ bio-high entropy alloy was developed utilizing thermodynamically parameters and calculations to fabricate a high efficiency biomaterial. Microstructural evolutions were characterized using X-ray diffraction (XRD), scanning electron microscope as well as energy-dispersive X-ray spectroscopy (EDS) techniques. Studies conducted on this alloy demonstrated the formation of a BCC solid solution without the presence of any intermetallic compounds. The results showed that the developed high entropy alloy exhibited the lowest elastic modulus compared to the control samples, which was reported as 115 GPa. The results of the nano-scratch test indicated a lower scratch depth and friction coefficient compared to the control samples for the bio-high entropy alloy. In addition, the Ti₃₅Nb₂₅Zr₁₅Mo₁₅V₁₀ alloy exhibited lower corrosion rate in three environments of PBS, NaCl, and H₂SO₄, in comparison to the control samples. The results of the shear punch test suggested better mechanical properties of the studied bio-high entropy alloy compared to the control samples. Overall, the investigation regarding the mechanical properties, corrosion resistance, and wear characteristics showed that the developed Ti₃₅Nb₂₅Zr₁₅Mo₁₅V₁₀ alloy has the significant potential to be used as a new metallic biomaterial, serving as a substitute for conventional Ti6Al4V and 316L SS alloys.</p>