Scholarlink

<p>Understanding the amount of cellulose present on the deep-sea floor is a major challenge in studying deep-sea ecosystems. However, conventional sugar analysis methods cannot directly address this question. Here, we propose a novel quantification methodology based on extraction with a heavy liquid followed by selective enzymatic hydrolysis. First, sediment samples undergo acid and alkaline pretreatment. Then, extraction is performed using density separation with a heavy liquid. The extracts contain not only cellulose but also minor amounts of light inorganic matter. Therefore, the extracts are further subjected to the selective hydrolysis by cellulase and β-glucosidase. During enzymatic hydrolysis, cellulose is converted into glucose, which is quantified using a commercial glucose assay kit. From the measured glucose content, the cellulose concentration in benthic sediment samples can be determined. While this method successfully quantified 1&#xa0;mg of artificially added cellulose in 200&#xa0;mg of spiked model deep-sea sediment, no native sediment samples have yet exhibited cellulose concentrations above the detection limit.</p><p></p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Challenges in extraction and quantification of cellulose from deep-sea sediments

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年10月29日

查看全文
  • Noriyuki Isobe,
  • Sachiko Kawada,
  • Yuto Kaku,
  • Kiyohiko Igarashi

摘要

Understanding the amount of cellulose present on the deep-sea floor is a major challenge in studying deep-sea ecosystems. However, conventional sugar analysis methods cannot directly address this question. Here, we propose a novel quantification methodology based on extraction with a heavy liquid followed by selective enzymatic hydrolysis. First, sediment samples undergo acid and alkaline pretreatment. Then, extraction is performed using density separation with a heavy liquid. The extracts contain not only cellulose but also minor amounts of light inorganic matter. Therefore, the extracts are further subjected to the selective hydrolysis by cellulase and β-glucosidase. During enzymatic hydrolysis, cellulose is converted into glucose, which is quantified using a commercial glucose assay kit. From the measured glucose content, the cellulose concentration in benthic sediment samples can be determined. While this method successfully quantified 1 mg of artificially added cellulose in 200 mg of spiked model deep-sea sediment, no native sediment samples have yet exhibited cellulose concentrations above the detection limit.