Scholarlink

<p>To address the challenges of high-density animal cell culture, this study developed a high-density culture system comprising a single-use bioreactor (SUB) with a nominal volume of 500 mL and a pulsed tangential flow filtration (ITF) unit. The reactor can be configured with two layers of 35&#xa0;mm diameter impellers—either double Elephant Ear (EE-EE) or Elephant Ear combined with Ribbon (EE-RB). The flow field characteristics were rigorously characterized through CFD simulations (60–240&#xa0;rpm; 90–480 mL) validated by experimental data. Engineering analysis revealed the system’s robust culture environment: <InlineEquation ID="IEq1"> <EquationSource Format="TEX">\({k}_{L}a\)</EquationSource> </InlineEquation> values ranging from 2 to 15&#xa0;h<sup>−1</sup> (60–180&#xa0;rpm; 200–400 mL; 30–150 mL/min aeration), <InlineEquation ID="IEq2"> <EquationSource Format="TEX">\(P/V\)</EquationSource> </InlineEquation> values from 0.1 to 10&#xa0;W/m<sup>3</sup>, and mixing times between 1 and 10&#xa0;s. Crucially, the system maintains a mild shear environment with an average shear strain rate (SSR) below 25&#xa0;s<sup>−1</sup>, fully within the physiological tolerance range for mammalian cells. This low-shear, high-mass-transfer design was validated through perfusion cultures of CHO and HEK293 cells. The system achieved exceptionally high cell densities of 8.32 × 10<sup>7</sup> cells/mL and 1.17 × 10<sup>8</sup> cells/mL, respectively, with cell viability consistently exceeding 90%, demonstrating its suitability for high-intensity biopharmaceutical production.</p> Graphical abstract <p></p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

CFD-aided design and hydrodynamic characterization of a single-use perfusion bioreactor for high density cell culture

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年6月3日

查看全文
  • Yongqiang Liu,
  • Qingfeng Gu,
  • Yu Liu,
  • Guoqian Xu,
  • Yingping Zhuang,
  • Meijin Guo,
  • Chao Li

摘要

To address the challenges of high-density animal cell culture, this study developed a high-density culture system comprising a single-use bioreactor (SUB) with a nominal volume of 500 mL and a pulsed tangential flow filtration (ITF) unit. The reactor can be configured with two layers of 35 mm diameter impellers—either double Elephant Ear (EE-EE) or Elephant Ear combined with Ribbon (EE-RB). The flow field characteristics were rigorously characterized through CFD simulations (60–240 rpm; 90–480 mL) validated by experimental data. Engineering analysis revealed the system’s robust culture environment: \({k}_{L}a\) values ranging from 2 to 15 h−1 (60–180 rpm; 200–400 mL; 30–150 mL/min aeration), \(P/V\) values from 0.1 to 10 W/m3, and mixing times between 1 and 10 s. Crucially, the system maintains a mild shear environment with an average shear strain rate (SSR) below 25 s−1, fully within the physiological tolerance range for mammalian cells. This low-shear, high-mass-transfer design was validated through perfusion cultures of CHO and HEK293 cells. The system achieved exceptionally high cell densities of 8.32 × 107 cells/mL and 1.17 × 108 cells/mL, respectively, with cell viability consistently exceeding 90%, demonstrating its suitability for high-intensity biopharmaceutical production.

Graphical abstract