Scholarlink

<p>Current therapies for prostate cancer are limited by toxicity and acquired resistance, motivating development of biocompatible nanotherapeutics. Here, <i>Solanum nigrum</i> L.-derived nanovesicles (SDNVs) were isolated and characterized, showing a mean diameter of 93.4 ± 0.2&#xa0;nm and a particle concentration of (4.2 ± 0.4) × 10<sup>11</sup>/mL. SDNV uptake, antitumor activity, and safety were assessed in PC-3 and RWPE-1 cells, while efficacy and biodistribution were further evaluated in a subcutaneous PC-3 xenograft mouse model following oral administration. SDNVs were readily internalized by PC-3 cells and suppressed viability, proliferation, and migration while inducing apoptosis. Senescence and cytotoxicity were not observed in RWPE-1 cells, indicating tumor-selective activity. The underlying mechanism was further investigated by transcriptome sequencing, followed by quantitative PCR, Western blotting, and pharmacologic rescue with the p53 inhibitor pifithrin-α (PFT-α). Transcriptomic analysis revealed 5058 differentially expressed genes, and further indicated activation of the p53/p21 axis and promoted cellular senescence in PC-3 cells after SDNV treatment. In vivo, oral SDNVs significantly reduced xenograft growth and showed no evident histopathologic toxicity in major organs. Collectively, SDNVs represent a plant-derived, biocompatible nanotherapeutic platform that selectively restrains prostate cancer growth via p53/p21-mediated senescence, supporting further development of senescence-targeting SDNV-based interventions.</p> Graphical abstract <p></p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Solanum nigrum L.-derived nanovesicles as novel nanotherapeutics suppressing prostate cancer progression via senescence-based antitumor activity

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年4月27日

查看全文
  • Miao Gan,
  • Yufei Feng,
  • Luhua Xu,
  • Zetao Chen,
  • Fanjia Zhong,
  • Na Liu,
  • Jiamei Huang,
  • Huizhen Zhang,
  • Jin Tu,
  • Chao Yang,
  • Rongfeng Yang,
  • Fengxia Lin

摘要

Current therapies for prostate cancer are limited by toxicity and acquired resistance, motivating development of biocompatible nanotherapeutics. Here, Solanum nigrum L.-derived nanovesicles (SDNVs) were isolated and characterized, showing a mean diameter of 93.4 ± 0.2 nm and a particle concentration of (4.2 ± 0.4) × 1011/mL. SDNV uptake, antitumor activity, and safety were assessed in PC-3 and RWPE-1 cells, while efficacy and biodistribution were further evaluated in a subcutaneous PC-3 xenograft mouse model following oral administration. SDNVs were readily internalized by PC-3 cells and suppressed viability, proliferation, and migration while inducing apoptosis. Senescence and cytotoxicity were not observed in RWPE-1 cells, indicating tumor-selective activity. The underlying mechanism was further investigated by transcriptome sequencing, followed by quantitative PCR, Western blotting, and pharmacologic rescue with the p53 inhibitor pifithrin-α (PFT-α). Transcriptomic analysis revealed 5058 differentially expressed genes, and further indicated activation of the p53/p21 axis and promoted cellular senescence in PC-3 cells after SDNV treatment. In vivo, oral SDNVs significantly reduced xenograft growth and showed no evident histopathologic toxicity in major organs. Collectively, SDNVs represent a plant-derived, biocompatible nanotherapeutic platform that selectively restrains prostate cancer growth via p53/p21-mediated senescence, supporting further development of senescence-targeting SDNV-based interventions.

Graphical abstract