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<p>This study investigates the optimisation of poly(3-hydroxybutyrate) (PHB) production by <i>Mycolicibacterium smegmatis</i> using sugarcane bagasse (SCB) hydrolysate as a low-cost, renewable carbon source. Key fermentation parameters including temperature, pH, agitation, inoculum size and nitrogen supplementation were optimised to enhance biomass growth and PHB accumulation. Under optimised conditions (37&#xa0;°C, pH 7, 100&#xa0;rpm, 3% inoculum), <i>M. smegmatis</i> achieved a maximum PHB content of 64% of DCW from SCB hydrolysate, corresponding to a PHB titre of ~ 1.0&#xa0;g L<sup>−1</sup> and a volumetric productivity of 0.014&#xa0;g L<sup>−1</sup> h<sup>−1</sup> after 72&#xa0;h of cultivation, with yeast extract identified as the most effective nitrogen source. Thin-layer chromatography (TLC) analysis demonstrated that <i>M. smegmatis</i> could not only co-utilise glucose and xylose simultaneously but preferentially consumed xylose, which is a major advantage when processing lignocellulosic biomasses, where xylose is abundant and typically underutilised by many microorganisms. Whole-genome sequencing with Oxford Nanopore Technologies (ONT) confirmed the presence of PHB production genes (<i>phbA</i>, <i>phbB</i>, <i>phbC</i>) and a xylose-utilisation pathway, supporting its metabolic capability. These findings establish <i>M. smegmatis</i> as a promising candidate for converting agricultural waste into biodegradable bioplastics, contributing to circular bioeconomy strategies.</p> Graphical abstract <p></p>

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Process optimisation and genomic analysis of poly(3-hydroxybutyrate) production by Mycolicibacterium smegmatis using sugarcane bagasse

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年4月2日

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  • Soulayma Hassan,
  • Christian Krohn,
  • Gerardo Aguilar Jr.,
  • Alexis Marshall,
  • Andrew S. Ball

摘要

This study investigates the optimisation of poly(3-hydroxybutyrate) (PHB) production by Mycolicibacterium smegmatis using sugarcane bagasse (SCB) hydrolysate as a low-cost, renewable carbon source. Key fermentation parameters including temperature, pH, agitation, inoculum size and nitrogen supplementation were optimised to enhance biomass growth and PHB accumulation. Under optimised conditions (37 °C, pH 7, 100 rpm, 3% inoculum), M. smegmatis achieved a maximum PHB content of 64% of DCW from SCB hydrolysate, corresponding to a PHB titre of ~ 1.0 g L−1 and a volumetric productivity of 0.014 g L−1 h−1 after 72 h of cultivation, with yeast extract identified as the most effective nitrogen source. Thin-layer chromatography (TLC) analysis demonstrated that M. smegmatis could not only co-utilise glucose and xylose simultaneously but preferentially consumed xylose, which is a major advantage when processing lignocellulosic biomasses, where xylose is abundant and typically underutilised by many microorganisms. Whole-genome sequencing with Oxford Nanopore Technologies (ONT) confirmed the presence of PHB production genes (phbA, phbB, phbC) and a xylose-utilisation pathway, supporting its metabolic capability. These findings establish M. smegmatis as a promising candidate for converting agricultural waste into biodegradable bioplastics, contributing to circular bioeconomy strategies.

Graphical abstract