Background <p> Tomato leaf curl New Delhi virus (ToLCNDV) causes severe economic losses and has a wide host range, infecting important crops such as members of the <i>Solanaceae</i> and <i>Cucurbitaceae</i> families. A rapid, sensitive and accurate visual detection method is urgently required for monitoring of the virus in the field and containing its spread.</p> Results <p> In this study, we integrated the recombinase-aid amplification (RAA) with the CRISPR/Cas12a system to develop a one-pot visual detection method for ToLCNDV by fluorescence monitoring. RAA primers were designed based on the analysis of conserved regions within reported ToLCNDV isolates. After optimization of the system, this detection method demonstrated its specific detection of ToLCNDV, particularly no cross-reaction to genetically closely related squash leaf curl China virus (SLCCNV) and showed excellent sensitivity, with the viral detection limit reaching 10¹ copies/µL. Evaluation of the method in twenty-eight field samples confirmed the consistent results with those from conventional polymerase chain reaction (PCR) detection. Twelve samples were selected for the validation of a one-pot RAA-CRISPR/Cas12a detection system, employing a field-based crude DNA extraction method. This method was found to be consistent with previous results, thereby confirming the feasibility of the method for field application.</p> Conclusion <p> We developed a one-pot visual detection system integrating CRISPR/Cas12a with RAA technology for the rapid, reliable, and cost-effective field diagnosis of ToLCNDV.</p> Graphical Abstract <p></p>