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Background <p>The S-adenosyl-L-methionine (SAM)-dependent methyltransferase SABATH gene family is integral to regulating plant growth, development, and stress responses. Despite tobacco being an economically important crop and a widely used model organism, a systematic characterization of its SABATH gene family remains lacking.</p> Results <p>In this study, 48 SABATH genes were identified in tobacco and phylogenetically classified into three clades based on their methyltransferase functions. Comprehensive analyses of gene structure, motif composition, and evolutionary patterns revealed substantial divergence among these NtSABATH genes, with their evolution primarily driven by purifying selection. Additionally, NtSABATHs are predicted to be regulated by various miRNAs and multiple transcription factors associated with diverse stress responses. Expression profiling based on public RNA-seq datasets and qRT-PCR analyses demonstrated distinct tissue-specific expression patterns for NtSABATHs, indicating their crucial roles in plant growth, development, and responses to various biotic and abiotic stresses. Notably, we confirmed the crucial role of <i>NtSABATH17</i> in enhancing tobacco’s resistance to black shank disease.</p> Conclusions <p>This study provides a comprehensive characterization of the NtSABATH gene family through phylogenetic analysis and gene expression profiling, revealing their multifaceted roles in plant development and stress adaptation. These findings establish a solid foundation for future functional studies of NtSABATH genes.</p> Graphical Abstract <p></p>

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Insight into the SABATH gene family in Nicotiana tabacum and functional analysis of NtSABATH17 in defense against black shank disease

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年4月6日

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  • Yiran Fu,
  • Haibin Zheng,
  • Zechao Qu,
  • Xinhua Tian,
  • Qiao Wang,
  • Xiaodong Xie,
  • Peng Lu,
  • Peijian Cao,
  • Jiemeng Tao,
  • Jianfeng Zhang,
  • Yangyang Li,
  • Jingjing Jin

摘要

Background

The S-adenosyl-L-methionine (SAM)-dependent methyltransferase SABATH gene family is integral to regulating plant growth, development, and stress responses. Despite tobacco being an economically important crop and a widely used model organism, a systematic characterization of its SABATH gene family remains lacking.

Results

In this study, 48 SABATH genes were identified in tobacco and phylogenetically classified into three clades based on their methyltransferase functions. Comprehensive analyses of gene structure, motif composition, and evolutionary patterns revealed substantial divergence among these NtSABATH genes, with their evolution primarily driven by purifying selection. Additionally, NtSABATHs are predicted to be regulated by various miRNAs and multiple transcription factors associated with diverse stress responses. Expression profiling based on public RNA-seq datasets and qRT-PCR analyses demonstrated distinct tissue-specific expression patterns for NtSABATHs, indicating their crucial roles in plant growth, development, and responses to various biotic and abiotic stresses. Notably, we confirmed the crucial role of NtSABATH17 in enhancing tobacco’s resistance to black shank disease.

Conclusions

This study provides a comprehensive characterization of the NtSABATH gene family through phylogenetic analysis and gene expression profiling, revealing their multifaceted roles in plant development and stress adaptation. These findings establish a solid foundation for future functional studies of NtSABATH genes.

Graphical Abstract