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Background <p>Edeine, a non-ribosomal antibiotic produced by <i>Brevibacillus brevis</i> X23, has a broad-spectrum antimicrobial activity against plant pathogens, but its low yield in wild-type strains limits its potential for agricultural applications. This study aimed to enhance edeine production by genetically engineering <i>B. brevis</i> X23.</p> Methods <p>Red/ET homologous recombination technology was used to construct engineered strain X23(Δ<i>abrB</i>)::P<sub><i>mwp</i></sub> by knocking out global negative regulator AbrB and replacing the natural promoter of the edeine biosynthesis gene cluster (<i>ede</i> BGC) with the strong P<sub><i>mwp</i></sub> promoter.</p> Results <p>Quantitative PCR revealed significantly increased <i>ede</i> BGC transcription levels in X23(Δ<i>abrB</i>)::P<sub><i>mwp</i></sub> compared to the wild-type strain. High-performance liquid chromatography–mass spectrometry (HPLC–MS) demonstrated a 10.1-fold increase in the edeine peak area with the final yield reaching 97.3 mg/L. In pot experiments for tobacco bacterial wilt (pathogen name <i>Ralstonia solanacearum</i>) control, X23(Δ<i>abrB</i>)::P<sub><i>mwp</i></sub> showed an efficacy of 82.9%, representing a 32.6% improvement over the wild-type strain (62.5%). The engineered strain also demonstrated an increased plate inhibition capacity of 20.5–60.9% against <i>Verticillium dahliae</i> Kleb, <i>Rhizoctonia solani</i>, and <i>Fusarium oxysporum</i> in cotton, indicating its potential application in crop protection.</p> Conclusions <p>Therefore, this study yielded an engineered strain with increased edeine production and enhanced biocontrol efficacy, contributing to the development of biological control methods for plant diseases.</p> Graphical Abstract <p></p>

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Construction of high-yielding edeine strains and an initial exploration of their control efficacy against crop pathogens

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年5月21日

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  • Liang Zhang,
  • Ziyue Chen,
  • Fei Xia,
  • Tianbo Liu,
  • Qingshu Liu,
  • Wu Chen

摘要

Background

Edeine, a non-ribosomal antibiotic produced by Brevibacillus brevis X23, has a broad-spectrum antimicrobial activity against plant pathogens, but its low yield in wild-type strains limits its potential for agricultural applications. This study aimed to enhance edeine production by genetically engineering B. brevis X23.

Methods

Red/ET homologous recombination technology was used to construct engineered strain X23(ΔabrB)::Pmwp by knocking out global negative regulator AbrB and replacing the natural promoter of the edeine biosynthesis gene cluster (ede BGC) with the strong Pmwp promoter.

Results

Quantitative PCR revealed significantly increased ede BGC transcription levels in X23(ΔabrB)::Pmwp compared to the wild-type strain. High-performance liquid chromatography–mass spectrometry (HPLC–MS) demonstrated a 10.1-fold increase in the edeine peak area with the final yield reaching 97.3 mg/L. In pot experiments for tobacco bacterial wilt (pathogen name Ralstonia solanacearum) control, X23(ΔabrB)::Pmwp showed an efficacy of 82.9%, representing a 32.6% improvement over the wild-type strain (62.5%). The engineered strain also demonstrated an increased plate inhibition capacity of 20.5–60.9% against Verticillium dahliae Kleb, Rhizoctonia solani, and Fusarium oxysporum in cotton, indicating its potential application in crop protection.

Conclusions

Therefore, this study yielded an engineered strain with increased edeine production and enhanced biocontrol efficacy, contributing to the development of biological control methods for plant diseases.

Graphical Abstract