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Background <p>Polysaccharides were extracted from the roots of <i>Scutellaria baicalensis</i> Georgi using hot water and ethanol precipitation method. The SBP-1 and SBP-2, were obtained after eluting from DE-52 column. The molecular weight, monosaccharide composition and linkage types of SBP-1 and SBP-2 were determined by the high performance gel permeation chromatography, high-performance anion exchange chromatography, gas chromatography–mass spectroscopy and NMR spectra.</p> Results <p>SBP-1 and SBP-2 were characterized as a homogenous polysaccharide with Mw of 80.5 and 25.8&#xa0;kDa, respectively. Moreover, there were seven linkage types in the SBP-1 and it was a type II arabinogalactan formed by a (1→6)-linked β-<span>d</span>-Gal<i>p</i> main chain. Whereas, except for the linkage types of α-<span>l</span>-Ara<i>f</i>-(1→, →5)-α-<span>l</span>-Ara<i>f</i>-(1→ and →3,5)-α-<span>l</span>-Ara<i>f</i>-(1→, the SBP-2 enriched with →3,4)-α-<span>d</span>-Gal<i>p</i>A-(1→, →4)-α-<span>d</span>-Gal<i>p</i>A-(1→ and →4)-α-<span>d</span>-Gal<i>p</i>A(OMe)-(1→, indicating the pectic type arabinogalacturonan structure. Moreover, both SBP-1 and SBP-2 exhibited immunoregulatory effects on RAW 264.7 cells by modulating the toll-like receptor (TLR) signaling pathway, as demonstrated through RNA-seq analysis, western blotting, immunoprecipitation (IP) experiments, and surface plasmon resonance (SPR) analysis.</p> Conclusions <p>These findings suggest an immunological basis for the therapeutic potential of <i>S. baicalensis</i>.</p> Graphical Abstract <p></p>

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Structure elucidation of arabinogalactan and arabinogalacturonan from the root of Scutellaria baicalensis Georgi and evaluation on their immunoregulating effects via TLR2 pathway

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年3月15日

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  • Xiaojun Li,
  • Rui Zhang,
  • Huizi Tang,
  • Yu Kou,
  • Yuan Yin,
  • Haiyang Guo,
  • Xue Zhang

摘要

Background

Polysaccharides were extracted from the roots of Scutellaria baicalensis Georgi using hot water and ethanol precipitation method. The SBP-1 and SBP-2, were obtained after eluting from DE-52 column. The molecular weight, monosaccharide composition and linkage types of SBP-1 and SBP-2 were determined by the high performance gel permeation chromatography, high-performance anion exchange chromatography, gas chromatography–mass spectroscopy and NMR spectra.

Results

SBP-1 and SBP-2 were characterized as a homogenous polysaccharide with Mw of 80.5 and 25.8 kDa, respectively. Moreover, there were seven linkage types in the SBP-1 and it was a type II arabinogalactan formed by a (1→6)-linked β-d-Galp main chain. Whereas, except for the linkage types of α-l-Araf-(1→, →5)-α-l-Araf-(1→ and →3,5)-α-l-Araf-(1→, the SBP-2 enriched with →3,4)-α-d-GalpA-(1→, →4)-α-d-GalpA-(1→ and →4)-α-d-GalpA(OMe)-(1→, indicating the pectic type arabinogalacturonan structure. Moreover, both SBP-1 and SBP-2 exhibited immunoregulatory effects on RAW 264.7 cells by modulating the toll-like receptor (TLR) signaling pathway, as demonstrated through RNA-seq analysis, western blotting, immunoprecipitation (IP) experiments, and surface plasmon resonance (SPR) analysis.

Conclusions

These findings suggest an immunological basis for the therapeutic potential of S. baicalensis.

Graphical Abstract