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<p><i>Siraitia grosvenorii,</i> commonly known as Luo Han Guo, is a medicinal and edible plant whose flowers contain bioactive polysaccharides with underexplored therapeutic potential. This study isolated a novel polysaccharide fraction (SGFP-2) from <i>Siraitia grosvenorii</i> flowers through DEAE-Crystarose Fast Flow chromatography. Structural analysis revealed SGFP-2 is a heteropolysaccharide with average molecular weight of 1.67 × 10<sup>5</sup>&#xa0;Da and composed of Rha, Glc, Gal, GlcA, GalA, Man, and Ara with a molar ratio of 8.17:1.54:60.06:3.41:5.37:3.54:15.44. Methylation analysis identified dominant glycosidic linkages of SGFP-2 as → 6)-Gal<i>p</i>-(1 → (30.42%), Gal<i>p</i>-(1 → (22.69%), Ara<i>f</i>-(1 → (18.29%), Rha<i>p</i>-(1 → (8.70%), → 3,6)-Gal<i>p</i>-(1 → (8.54%). Scanning electron microscopy and Congo red staining results showed that the network structure of SGFP-2 was lamellar without trihelix conformation. In vitro experiments have revealed that SGFP-2 possesses lipid-binding capacity, bile salt adsorption properties, and potent inhibitory activity against crucial enzymes involved in glucose-lipid metabolism, specifically pancreatic lipase, cholesterol esterase, α-amylase, and α-glucosidase. These findings suggest that SGFP-2 demonstrates potential&#xa0;hypolipidemic and hypoglycemic effects in vitro. This study provides preliminary evidence to support further development and utilization of <i>Siraitia grosvenorii</i> flower polysaccharides.</p> Graphical Abstract <p></p>

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Structural characterization and in vitro hypolipidemic and hypoglycemic activities of a novel neutral polysaccharide from Siraitia grosvenorii (Luo Han Guo) flowers

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年3月6日

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  • Hao Teng,
  • Limei Qiu,
  • Zhizhi Huang,
  • Sam Al-Dalali,
  • Cailin Li,
  • Aiying Xu,
  • Zhigui He

摘要

Siraitia grosvenorii, commonly known as Luo Han Guo, is a medicinal and edible plant whose flowers contain bioactive polysaccharides with underexplored therapeutic potential. This study isolated a novel polysaccharide fraction (SGFP-2) from Siraitia grosvenorii flowers through DEAE-Crystarose Fast Flow chromatography. Structural analysis revealed SGFP-2 is a heteropolysaccharide with average molecular weight of 1.67 × 105 Da and composed of Rha, Glc, Gal, GlcA, GalA, Man, and Ara with a molar ratio of 8.17:1.54:60.06:3.41:5.37:3.54:15.44. Methylation analysis identified dominant glycosidic linkages of SGFP-2 as → 6)-Galp-(1 → (30.42%), Galp-(1 → (22.69%), Araf-(1 → (18.29%), Rhap-(1 → (8.70%), → 3,6)-Galp-(1 → (8.54%). Scanning electron microscopy and Congo red staining results showed that the network structure of SGFP-2 was lamellar without trihelix conformation. In vitro experiments have revealed that SGFP-2 possesses lipid-binding capacity, bile salt adsorption properties, and potent inhibitory activity against crucial enzymes involved in glucose-lipid metabolism, specifically pancreatic lipase, cholesterol esterase, α-amylase, and α-glucosidase. These findings suggest that SGFP-2 demonstrates potential hypolipidemic and hypoglycemic effects in vitro. This study provides preliminary evidence to support further development and utilization of Siraitia grosvenorii flower polysaccharides.

Graphical Abstract