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Background <p>DnaJA proteins, a prominent subfamily of the DnaJ family, function as molecular chaperones that respond to various external stresses. Extensive studies on the DnaJ family have been conducted in plants. However, research on this subfamily in tobacco remains relatively scarce.</p> Results <p>In this study, we identified 24 DnaJA genes in tobacco, and classified them into three individual groups. A comprehensive analysis based on gene structure, motif composition, and evolutionary pattern revealed the divergence of tobacco DnaJA genes. For the evolution of the <i>NtDnaJA</i> genes, purification selection was the major factor. In addition, the potential regulatory network unveiled that <i>NtDnaJA</i>s could be regulated by miRNAs and various transcription factors associated with diverse stress responses. Through expression pattern analysis of public RNA-seq datasets and qRT-PCR experiments, it was observed that many <i>NtDnaJAs</i> displayed tissue-specific expression and might play significant roles in different biotic and abiotic stresses. Additionally, the pivotal role of <i>NtDnaJA3</i> in boosting plant drought resistance was confirmed.</p> Conclusions <p>This study provides important perspectives on the evolution of <i>NtDnaJA</i> genes and their involvement in stress responses, laying the groundwork for future research into the roles of DnaJA regulatory genes in tobacco.</p> Graphical Abstract <p></p>

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Genome-wide analysis of DnaJA proteins in Nicotiana tabacum reveals NtDnaJA3 responses to drought stress

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年1月7日

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  • Qiao Wang,
  • Lijun Meng,
  • Zechao Qu,
  • Huan Su,
  • Jiemeng Tao,
  • Peng Lu,
  • Chenfeng Hua,
  • Jinbang Wang,
  • Jianfeng Zhang,
  • Peijian Cao,
  • Jingjing Jin

摘要

Background

DnaJA proteins, a prominent subfamily of the DnaJ family, function as molecular chaperones that respond to various external stresses. Extensive studies on the DnaJ family have been conducted in plants. However, research on this subfamily in tobacco remains relatively scarce.

Results

In this study, we identified 24 DnaJA genes in tobacco, and classified them into three individual groups. A comprehensive analysis based on gene structure, motif composition, and evolutionary pattern revealed the divergence of tobacco DnaJA genes. For the evolution of the NtDnaJA genes, purification selection was the major factor. In addition, the potential regulatory network unveiled that NtDnaJAs could be regulated by miRNAs and various transcription factors associated with diverse stress responses. Through expression pattern analysis of public RNA-seq datasets and qRT-PCR experiments, it was observed that many NtDnaJAs displayed tissue-specific expression and might play significant roles in different biotic and abiotic stresses. Additionally, the pivotal role of NtDnaJA3 in boosting plant drought resistance was confirmed.

Conclusions

This study provides important perspectives on the evolution of NtDnaJA genes and their involvement in stress responses, laying the groundwork for future research into the roles of DnaJA regulatory genes in tobacco.

Graphical Abstract