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Introduction <p>The problem of wound healing and the availability of effective and safe agents with regenerative properties is critically important today. The aim of the study was to develop an algorithm for evaluating products with regenerative potential using a set of in vitro and in vivo experimental models.</p> Methods <p>In vitro research included methods of working with cell culture. Studies were conducted using cell lines: HaCat, MAEС, 3T3-A31. In vivo research was conducted on Wistar rats with simulation of the pathological condition of a cut wound and the application of experimental samples and the following assessment of a set of indicators.</p> Results <p>As a result of using a comprehensive approach to assess the effectiveness of agents with regenerative activity, it was shown that the spray and gel samples, the main component of which is collagen, with decamethoxine, particles of silver sulfadiazine, hyaluronic acid, and additionally a set of amino acids in a spray, were shown to cause a statistically significant increase in the number of live cells at certain concentrations in vitro and increase the speed of restoration of the damaged monolayer of endothelial cells. According to the results of the speed of healing of the skin in the area of ​​the simulated cut wound, a statistically significant increase in the speed of healing from 10 to 19% with the use of the spray and from 5 to 18% with the use of the gel in comparison with the animals of the control group (р≤0.05 and <i>p</i> ≤ 0.01). The evaluation of the results of the morphological analysis showed that with repeated application of the spray or gel, a significant reduction in the area of ​​the wound is observed already on the 8th day with faster healing compared to the control. Already from the 8th day, inflammatory changes and the area of ​​necrotic in the experimental groups significantly decreased.</p> Conclusion <p>A wound healing assessment model was developed and tested, which is convenient for monitoring the wound healing process and evaluating treatment outcomes. The efficacy and potential role in accelerating wound healing of new gel and spray products for external use was shown.</p> Clinical trial number <p>Not applicable.</p>

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Assessment of the efficiency of new regenerative gel and spray in experimental model systems

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年4月15日

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  • Natalia Bezdieniezhnykh,
  • Oleksandra Lykhova,
  • Maryna Borshchevska,
  • Andriy Goy,
  • Kyrylo Saulenko,
  • Tamara Kozak,
  • Dmytro Kukurudza,
  • Yevgen Kruglov,
  • Genadiy Borshchevskiy,
  • Liubov Buchynska

摘要

Introduction

The problem of wound healing and the availability of effective and safe agents with regenerative properties is critically important today. The aim of the study was to develop an algorithm for evaluating products with regenerative potential using a set of in vitro and in vivo experimental models.

Methods

In vitro research included methods of working with cell culture. Studies were conducted using cell lines: HaCat, MAEС, 3T3-A31. In vivo research was conducted on Wistar rats with simulation of the pathological condition of a cut wound and the application of experimental samples and the following assessment of a set of indicators.

Results

As a result of using a comprehensive approach to assess the effectiveness of agents with regenerative activity, it was shown that the spray and gel samples, the main component of which is collagen, with decamethoxine, particles of silver sulfadiazine, hyaluronic acid, and additionally a set of amino acids in a spray, were shown to cause a statistically significant increase in the number of live cells at certain concentrations in vitro and increase the speed of restoration of the damaged monolayer of endothelial cells. According to the results of the speed of healing of the skin in the area of ​​the simulated cut wound, a statistically significant increase in the speed of healing from 10 to 19% with the use of the spray and from 5 to 18% with the use of the gel in comparison with the animals of the control group (р≤0.05 and p ≤ 0.01). The evaluation of the results of the morphological analysis showed that with repeated application of the spray or gel, a significant reduction in the area of ​​the wound is observed already on the 8th day with faster healing compared to the control. Already from the 8th day, inflammatory changes and the area of ​​necrotic in the experimental groups significantly decreased.

Conclusion

A wound healing assessment model was developed and tested, which is convenient for monitoring the wound healing process and evaluating treatment outcomes. The efficacy and potential role in accelerating wound healing of new gel and spray products for external use was shown.

Clinical trial number

Not applicable.