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Background <p>Several animal protocols have been designed to model binge eating disorder (BED) and elucidate its neurological basis. A wide variety of factors, including the frequency of access to palatable food and a previous history of caloric restriction, have been modulated to induce binge eating intake. The aims of this work were to compare the effects of these two factors on the development of binge eating behaviour and to analyse possible alterations in neurotransmitter markers in the striatum.</p> Methods <p>We performed a binge eating test in male and female C57BL/6J mice, which consisted of 2&#xa0;h of limited access to a high-fat high-sucrose (HFHS) diet in a sated state with <i>ad libitum</i> access to a control diet and water. Mice were subjected to either daily or intermittent (every other day) 2-h limited access to the HFHS, with or without previous episodes of caloric restriction. Dopaminergic, glutamatergic and GABAergic markers were analysed in the striatum by RT–qPCR. Behavioural and biomolecular differences between groups were analysed by one-way ANOVA for multiple comparisons.</p> Results <p>Both male and female mice developed binge eating behaviour following a 2-h HFHS access paradigm, while the frequency of exposure and history of caloric restriction did not affect the BE response. However, the combination of caloric restriction and intermittent access to HFHS induced an early BE response in females, with no compensatory decrease in chow intake and no weight changes in either sex. Moreover, we observed differences in the modulation of <i>Drd2</i> expression between animals subjected to binge eating and those with <i>ad libitum</i> access to HFHS, independent of sex.</p> Conclusions <p>This work is the first to compare the effects of the frequency of exposure to HFHS and a history of caloric restriction on binge eating behaviour in both male and female mice within a single experimental setting. The results do not reveal any major effects on binge eating behaviour but highlight the combination of both conditions for translational applications since any compensatory process is observed. Moreover, the results underscore a role of striatal <i>Drd2</i> expression as a key factor involved in the course of BED.</p>

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Influence of caloric restriction history, intermittent exposure to palatable food and sex in a binge eating model in C57BL/6J mice

  • 期刊论文

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  • 发表日期：

    2025年12月23日

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  • Nuria Morales-Puerto,
  • Clémence Fayt,
  • Chloé Tezenas du Montcel,
  • Amandine Everard

摘要

Background

Several animal protocols have been designed to model binge eating disorder (BED) and elucidate its neurological basis. A wide variety of factors, including the frequency of access to palatable food and a previous history of caloric restriction, have been modulated to induce binge eating intake. The aims of this work were to compare the effects of these two factors on the development of binge eating behaviour and to analyse possible alterations in neurotransmitter markers in the striatum.

Methods

We performed a binge eating test in male and female C57BL/6J mice, which consisted of 2 h of limited access to a high-fat high-sucrose (HFHS) diet in a sated state with ad libitum access to a control diet and water. Mice were subjected to either daily or intermittent (every other day) 2-h limited access to the HFHS, with or without previous episodes of caloric restriction. Dopaminergic, glutamatergic and GABAergic markers were analysed in the striatum by RT–qPCR. Behavioural and biomolecular differences between groups were analysed by one-way ANOVA for multiple comparisons.

Results

Both male and female mice developed binge eating behaviour following a 2-h HFHS access paradigm, while the frequency of exposure and history of caloric restriction did not affect the BE response. However, the combination of caloric restriction and intermittent access to HFHS induced an early BE response in females, with no compensatory decrease in chow intake and no weight changes in either sex. Moreover, we observed differences in the modulation of Drd2 expression between animals subjected to binge eating and those with ad libitum access to HFHS, independent of sex.

Conclusions

This work is the first to compare the effects of the frequency of exposure to HFHS and a history of caloric restriction on binge eating behaviour in both male and female mice within a single experimental setting. The results do not reveal any major effects on binge eating behaviour but highlight the combination of both conditions for translational applications since any compensatory process is observed. Moreover, the results underscore a role of striatal Drd2 expression as a key factor involved in the course of BED.