Scholarlink

Background <p><i>Biomphalaria straminea</i>, an intermediate host of <i>Schistosoma mansoni</i>, is originally native to Brazil but has invaded southern China since 1974. Nowadays, increasing human mobility raises the risk of <i>S. mansoni</i> dissemination. Therefore, this study aims to elucidate the genetic variation and structure of <i>B. straminea</i> in China and develop molecular tools for tracing its geographic origins, which could aid in schistosomiasis prevention and control.</p> Methods <p>We collected 290 <i>B. straminea</i> individuals from Shenzhen City (GDSZ, <i>n</i> = 171), Dongguan City (GDDG, <i>n</i> = 65), and Hong Kong (HK, <i>n</i> = 54). Double digest restriction associated DNA (ddRAD) sequencing was applied to genotype the samples. A subset of single nucleotide polymorphisms (SNPs) was validated by the Sequenom MassARRAY iPLEX assay. The MaxEnt model was employed to predict suitable habitats for <i>B. straminea</i> in China under current and future climate conditions.</p> Results <p>Analysis of ddRAD sequencing data led to the identification of 80 high-confidence SNPs. <i>B. straminea</i> from GDSZ exhibited higher genetic diversity than those from other locations. The total observed heterozygosity (<i>Ho</i> = 0.35) was higher than the total expected heterozygosity (<i>He</i> = 0.26), resulting in a&#xa0;negative inbreeding coefficient (<i>Fis</i> = −&#xa0;0.35), indicating that outbreeding has dominated the recent genetic history of <i>B. straminea</i>. Pairwise genetic distance (<i>Fst</i> &lt; 0.05) and number of effective migrants (<i>Nm</i> &gt; 4) indicated low genetic differentiation. The populations in GDSZ, GDDG and HK were genetically similar, with the first two being more closely related. Three high-quality SNPs displayed distinct geographical population specificity and could serve as geographically specific SNP markers. The MaxEnt model predicted an expansion of suitable habitats for <i>B. straminea</i> in China under future climate conditions. High invasion risk in Hainan Province, Guangxi Zhuang Autonomous Region, and Taiwan Province warrants attention.</p> Conclusions <p>This study provides the first genome-wide insights into the population structure and genetic diversity of <i>B. straminea</i> in China. The populations are genetically similar, suggesting a common invasion source. Applying the geographically specific SNPs could enable rapid prediction of the geographic origin of <i>B. straminea</i> in future invasion events. Future climate conditions are likely to facilitate the spread of <i>B. straminea</i>, increasing the risk of schistosomiasis transmission in China.</p> Graphical abstract <p></p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Invasive patterns of Biomphalaria straminea revealed by genetic mapping in the Greater Bay Area, China

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年1月15日

查看全文
  • Yue Hu,
  • Hui Huang,
  • Min-Yu Zhou,
  • Yun-Fei Zhou,
  • Hai-Mo Shen,
  • Jun-Hu Chen,
  • Zhi-Yue Lv

摘要

Background

Biomphalaria straminea, an intermediate host of Schistosoma mansoni, is originally native to Brazil but has invaded southern China since 1974. Nowadays, increasing human mobility raises the risk of S. mansoni dissemination. Therefore, this study aims to elucidate the genetic variation and structure of B. straminea in China and develop molecular tools for tracing its geographic origins, which could aid in schistosomiasis prevention and control.

Methods

We collected 290 B. straminea individuals from Shenzhen City (GDSZ, n = 171), Dongguan City (GDDG, n = 65), and Hong Kong (HK, n = 54). Double digest restriction associated DNA (ddRAD) sequencing was applied to genotype the samples. A subset of single nucleotide polymorphisms (SNPs) was validated by the Sequenom MassARRAY iPLEX assay. The MaxEnt model was employed to predict suitable habitats for B. straminea in China under current and future climate conditions.

Results

Analysis of ddRAD sequencing data led to the identification of 80 high-confidence SNPs. B. straminea from GDSZ exhibited higher genetic diversity than those from other locations. The total observed heterozygosity (Ho = 0.35) was higher than the total expected heterozygosity (He = 0.26), resulting in a negative inbreeding coefficient (Fis = − 0.35), indicating that outbreeding has dominated the recent genetic history of B. straminea. Pairwise genetic distance (Fst < 0.05) and number of effective migrants (Nm > 4) indicated low genetic differentiation. The populations in GDSZ, GDDG and HK were genetically similar, with the first two being more closely related. Three high-quality SNPs displayed distinct geographical population specificity and could serve as geographically specific SNP markers. The MaxEnt model predicted an expansion of suitable habitats for B. straminea in China under future climate conditions. High invasion risk in Hainan Province, Guangxi Zhuang Autonomous Region, and Taiwan Province warrants attention.

Conclusions

This study provides the first genome-wide insights into the population structure and genetic diversity of B. straminea in China. The populations are genetically similar, suggesting a common invasion source. Applying the geographically specific SNPs could enable rapid prediction of the geographic origin of B. straminea in future invasion events. Future climate conditions are likely to facilitate the spread of B. straminea, increasing the risk of schistosomiasis transmission in China.

Graphical abstract