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Background <p>Phytate is the primary phosphorus storage molecule of plants and plays a major role in animal nutrition. To enhance phosphate availability and absorption in livestock, and to reduce eutrophication by liquid manure, bacterial phytases are often added to animal feed. The dephosphorylated form of phytate, the polyol <i>myo</i>-inositol (<i>myo</i>-Ins) with multiple functions in eukaryotes, is metabolized by approximately 30% of all bacterial species.</p> Results <p>Here, we employed a culturomics approach to identify possible metabolic interactions between phytase-producing and <i>myo</i>-Ins degrading bacteria in intestinal samples from pigs. Selective cultivation revealed an unexpectedly high abundance of <i>myo</i>-Ins degrading bacteria, suggesting substantial phytate dephosphorylation in the pig gut. Phytase activity assays performed on gut isolates showed a high degree of variability, suggesting the presence of a diverse set of phytases yet to be characterized. Furthermore, using supernatants of phytase-positive gut strains cultivated in the presence of phytate, we observed cross-feeding of <i>myo</i>-Ins from phytase producers to phytase-negative strains, including the pathogen <i>Salmonella enterica</i> serovar Typhimurium.</p> Conclusions <p>The data demonstrate that a wide range of commensal bacteria can potentially benefit from phytase activity by utilizing <i>myo</i>-Ins, released through phytate hydrolysis, as a growth substrate.</p> <p><MediaObject ID="MOESM7"> <VideoObject FileRef="MediaObjects/40168_2025_2313_MOESM7_ESM.mp4" VideoID="8nus4SAF1X3z7QJk2qNbMB"> <Caption Language="En" xml:lang="en"> <CaptionContent> <p>Video Abstract</p> </CaptionContent> </Caption> </VideoObject> </MediaObject></p>

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A culturomics approach reveals cross-feeding capacity of intestinal pig bacteria upon release of inositol from phytate

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年1月21日

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  • Lena-Sophie Paul,
  • Michael Weber,
  • Stefanie Wagner,
  • Thilo M. Fuchs

摘要

Background

Phytate is the primary phosphorus storage molecule of plants and plays a major role in animal nutrition. To enhance phosphate availability and absorption in livestock, and to reduce eutrophication by liquid manure, bacterial phytases are often added to animal feed. The dephosphorylated form of phytate, the polyol myo-inositol (myo-Ins) with multiple functions in eukaryotes, is metabolized by approximately 30% of all bacterial species.

Results

Here, we employed a culturomics approach to identify possible metabolic interactions between phytase-producing and myo-Ins degrading bacteria in intestinal samples from pigs. Selective cultivation revealed an unexpectedly high abundance of myo-Ins degrading bacteria, suggesting substantial phytate dephosphorylation in the pig gut. Phytase activity assays performed on gut isolates showed a high degree of variability, suggesting the presence of a diverse set of phytases yet to be characterized. Furthermore, using supernatants of phytase-positive gut strains cultivated in the presence of phytate, we observed cross-feeding of myo-Ins from phytase producers to phytase-negative strains, including the pathogen Salmonella enterica serovar Typhimurium.

Conclusions

The data demonstrate that a wide range of commensal bacteria can potentially benefit from phytase activity by utilizing myo-Ins, released through phytate hydrolysis, as a growth substrate.

Video Abstract