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Background <p>The gut virome plays a pivotal role in shaping the host’s microbiota. However, gut viruses across different mammalian models, and their connections with the human gut microbiota remain largely unknown.</p> Results <p>We identified 977 high-confidence species-level viral operational taxonomic units (vOTUs) in mice (hcMGV), 12,896 in pigs (hcPGV), and 1480 in cynomolgus macaques (hcCMGV) from metagenomes, respectively. Clustering these vOTUs at approximately genus level uncovered novel clades with high prevalence across animal guts (&gt; = 60%). In particular, crAss-like phages and <i>cas</i>-harboring jumbophages were characterized. Comparative analysis revealed that hcCMGV had a closer relationship with hcPGV than hcMGV, despite the animal-specific characteristics, and that 55.88% hcCMGV had connections with the human microbiota.</p> Conclusions <p>Our findings shed light on the diversity of gut viruses across these three animals, contributing to future gut microbial studies using model animals.</p> <p><MediaObject ID="MOESM5"> <VideoObject FileRef="MediaObjects/40168_2025_2144_MOESM5_ESM.mp4" VideoID="2sLt9pZ5RDGzh1-BPpdZZD"> <Caption Language="En" xml:lang="en"> <CaptionContent> <p>Video Abstract</p> </CaptionContent> </Caption> </VideoObject> </MediaObject></p>

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Metagenomic analysis reveals gut phage diversity across three mammalian models

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年6月20日

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  • Menghao Yu,
  • Yunmeng Chu,
  • Yongming Wang,
  • Luofei Mo,
  • Xin Tan,
  • Shun Guo,
  • Shengjian Yuan,
  • Yingfei Ma

摘要

Background

The gut virome plays a pivotal role in shaping the host’s microbiota. However, gut viruses across different mammalian models, and their connections with the human gut microbiota remain largely unknown.

Results

We identified 977 high-confidence species-level viral operational taxonomic units (vOTUs) in mice (hcMGV), 12,896 in pigs (hcPGV), and 1480 in cynomolgus macaques (hcCMGV) from metagenomes, respectively. Clustering these vOTUs at approximately genus level uncovered novel clades with high prevalence across animal guts (> = 60%). In particular, crAss-like phages and cas-harboring jumbophages were characterized. Comparative analysis revealed that hcCMGV had a closer relationship with hcPGV than hcMGV, despite the animal-specific characteristics, and that 55.88% hcCMGV had connections with the human microbiota.

Conclusions

Our findings shed light on the diversity of gut viruses across these three animals, contributing to future gut microbial studies using model animals.

Video Abstract