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Background <p>Interactions between the nasal epithelium, commensal nasal microbiota, and respiratory pathogens play a key role in respiratory infections. Currently, there is a lack of experimental models to study such interactions under defined in vitro conditions. Here, we developed a porcine nasal organoid (PNO) system from nasal tissue of pigs as well as from cytological brushes.</p> Results <p>PNOs exhibited similar structure and cell types to the nasal mucosa, as evaluated by immunostaining. PNOs were inoculated with porcine commensal strains of <i>Moraxella pluranimalium</i>,<i> Rothia nasimurium</i>, and the pathobiont <i>Glaesserella parasuis</i> for examining host-commensal-pathogen interactions. All strains adhered to the PNOs, although at different levels. <i>M. pluranimalium</i> and <i>G. parasuis</i> strains stimulated the production of proinflammatory cytokines, whereas <i>R. nasimurium</i> induced the production of IFNγ and diminished the proinflammatory effect of the other strains.</p> Conclusions <p>Overall, PNOs mimic the in vivo nasal mucosa and can be useful to perform host-microbe interaction studies.</p> <p><MediaObject ID="MOESM3"> <VideoObject FileRef="MediaObjects/40168_2025_2088_MOESM3_ESM.mp4" VideoID="2MyHx2G-nK6oeC4P7Gx6p1"> <Caption Language="En" xml:lang="en"> <CaptionContent> <p>Video Abstract</p> </CaptionContent> </Caption> </VideoObject> </MediaObject></p>

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Porcine nasal organoids to model interactions between the swine nasal microbiota and the host

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年5月22日

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  • Laura Bonillo-Lopez,
  • Noelia Carmona-Vicente,
  • Ferran Tarrés-Freixas,
  • Karl Kochanowski,
  • Jorge Martínez,
  • Mònica Perez,
  • Marina Sibila,
  • Florencia Correa-Fiz,
  • Virginia Aragon

摘要

Background

Interactions between the nasal epithelium, commensal nasal microbiota, and respiratory pathogens play a key role in respiratory infections. Currently, there is a lack of experimental models to study such interactions under defined in vitro conditions. Here, we developed a porcine nasal organoid (PNO) system from nasal tissue of pigs as well as from cytological brushes.

Results

PNOs exhibited similar structure and cell types to the nasal mucosa, as evaluated by immunostaining. PNOs were inoculated with porcine commensal strains of Moraxella pluranimalium, Rothia nasimurium, and the pathobiont Glaesserella parasuis for examining host-commensal-pathogen interactions. All strains adhered to the PNOs, although at different levels. M. pluranimalium and G. parasuis strains stimulated the production of proinflammatory cytokines, whereas R. nasimurium induced the production of IFNγ and diminished the proinflammatory effect of the other strains.

Conclusions

Overall, PNOs mimic the in vivo nasal mucosa and can be useful to perform host-microbe interaction studies.

Video Abstract