Scholarlink

Background <p>Therapeutic management of prostate cancer remains challenging. Prostatic artery chemoembolization (PACE), an advanced minimally invasive technique derived from prostatic artery embolization (PAE), has shown promising potential in controling tumor growth and alleviating symptoms such as hematuria and lower urinary tract obstruction in prostate cancer, and has garnered widespread attention in recent research. However, preclinical progress is hindered by the limitations of existing animal models, and the development of a simple and reliable animal model to better understand the endovascular interventional treatment of prostate cancer is necessary.</p> Objectives <p>To develop and characterize a novel xenograft model of human prostate cancer in the rabbit auricle as a practical platform for experimental research on endovascular embolization techniques.</p> Materials and methods <p>Fresh human PC3 cell suspension (0.1&#xa0;ml containing ~ 9 × 10<sup>6</sup> cells) was injected subcutaneously into each auricle of 15 immunosuppressed New Zealand white rabbits. Tumor growth was measured periodically, and volume doubling time (VDT) was calculated. Digital subtraction angiography was performed via the central auricular artery when tumors were greater than 150&#xa0;mm<sup>3</sup>. Subsequently, histopathological and immunohistochemical analyses were conducted. Repeated-measures ANOVA was used to assess the significance of differences in the VDT of auricle tumors within-rabbits and between rabbits.</p> Results <p>The model achieved a high tumor take-rate of 86% (26/30 auricles). Angiography revealed distinct tumor-feeding vessels and uniform ring-like staining. Histopathology confirmed invasive growth of poorly differentiated, atypical tumor cells with high mitotic activity and no obvious necrosis. Immunohistochemistry showed high Ki67 expression (82.43 ± 3.68)%, indicating active proliferation. During the exponential growth stage, the mean VDT was 6.4 ± 1.8&#xa0;days. Repeated-measures ANOVA revealed significant variations in VDT among the rabbit subjects (<i>P</i> &lt; 0.05); moreover, the VDT did not significantly differ between the left and right ears of each rabbit (<i>P</i> &gt; 0.05), thereby reinforcing the consistency of the model employed.</p> Conclusions <p>The rabbit auricle PC3 xenograft model is a valuable preclinical platform. It effectively simulates human prostate cancer with high reproducibility and clear vasculature, providing a foundational tool for subsequent investigations into the efficacy of endovascular therapies, such as PAE and PACE.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Development of a novel rabbit auricle xenograft model of human PC3 prostate cancer

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年3月9日

查看全文
  • Xilong Yu,
  • Heng Zhang,
  • Jinlong Zhang,
  • Yan Wang,
  • Jieyu Yan,
  • Bing Yuan,
  • Maoqiang Wang

摘要

Background

Therapeutic management of prostate cancer remains challenging. Prostatic artery chemoembolization (PACE), an advanced minimally invasive technique derived from prostatic artery embolization (PAE), has shown promising potential in controling tumor growth and alleviating symptoms such as hematuria and lower urinary tract obstruction in prostate cancer, and has garnered widespread attention in recent research. However, preclinical progress is hindered by the limitations of existing animal models, and the development of a simple and reliable animal model to better understand the endovascular interventional treatment of prostate cancer is necessary.

Objectives

To develop and characterize a novel xenograft model of human prostate cancer in the rabbit auricle as a practical platform for experimental research on endovascular embolization techniques.

Materials and methods

Fresh human PC3 cell suspension (0.1 ml containing ~ 9 × 106 cells) was injected subcutaneously into each auricle of 15 immunosuppressed New Zealand white rabbits. Tumor growth was measured periodically, and volume doubling time (VDT) was calculated. Digital subtraction angiography was performed via the central auricular artery when tumors were greater than 150 mm3. Subsequently, histopathological and immunohistochemical analyses were conducted. Repeated-measures ANOVA was used to assess the significance of differences in the VDT of auricle tumors within-rabbits and between rabbits.

Results

The model achieved a high tumor take-rate of 86% (26/30 auricles). Angiography revealed distinct tumor-feeding vessels and uniform ring-like staining. Histopathology confirmed invasive growth of poorly differentiated, atypical tumor cells with high mitotic activity and no obvious necrosis. Immunohistochemistry showed high Ki67 expression (82.43 ± 3.68)%, indicating active proliferation. During the exponential growth stage, the mean VDT was 6.4 ± 1.8 days. Repeated-measures ANOVA revealed significant variations in VDT among the rabbit subjects (P < 0.05); moreover, the VDT did not significantly differ between the left and right ears of each rabbit (P > 0.05), thereby reinforcing the consistency of the model employed.

Conclusions

The rabbit auricle PC3 xenograft model is a valuable preclinical platform. It effectively simulates human prostate cancer with high reproducibility and clear vasculature, providing a foundational tool for subsequent investigations into the efficacy of endovascular therapies, such as PAE and PACE.