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Background <p>To assess the impact of oil overlay during maturation of immature oocytes in <i>vitro</i> in our optimized liquid culture system.</p> Methods <p>We conducted comparative analyses of pH, osmolality, and the levels of melatonin, FSH, E2, and hCG in the culture medium between oil-covered group (OC group) and non-oil-covered group (non-OC group). Furthermore, germinal vesicle (GV) oocytes were collected and matured into MII phase in our culture medium for 24&#xa0;h. Subsequently, levels of ROS, SOD, MDA, and LDH were tested in OC group and non-OC group using either microplate assays or chemical fluorescence techniques. Finally, a total of 2432 immature oocytes obtained from controlled ovarian hyperstimulation (COH) cycles underwent in <i>vitro</i> maturation (IVM) in culture media with or without oil supplementation (1380 and 1052 oocytes, respectively), followed by insemination and embryo culture. The IVM prognostic indicators were assessed and compared between OC group and non-OC group.</p> Results <p>There were no statistically significant differences in pH and osmolality values between the OC group and the non-OC group. However, the concentrations of melatonin, FSH, E2, and hCG in non-OC group were significantly elevated in the non-OC group compared to the OC group. Regarding oxidative stress parameters, levels of ROS, MDA, and LDH in the culture medium of the non-OC group were lower than those in the OC group, while SOD levels were higher in the non-OC group. Furthermore, the clinical outcome assessments revealed significantly higher rates of maturation, cleavage, and blastocyst formation in the non-OC group compared to the OC group, with no discernible difference in fertilization rates between the two groups.</p> Conclusion <p>Omission of mineral oil overlay enhances hormone bioavailability, diminishes oxidative stress, and significantly improves oocyte maturation and blastocyst formation rates in IVM culture.</p>

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Impact of mineral oil overlay on immature oocyte maturation in an optimized in vitro culture system

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年1月30日

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  • Jing Peng,
  • Yan Hao,
  • Zhaojuan Yu,
  • Beili Chen,
  • Zhiguo Zhang

摘要

Background

To assess the impact of oil overlay during maturation of immature oocytes in vitro in our optimized liquid culture system.

Methods

We conducted comparative analyses of pH, osmolality, and the levels of melatonin, FSH, E2, and hCG in the culture medium between oil-covered group (OC group) and non-oil-covered group (non-OC group). Furthermore, germinal vesicle (GV) oocytes were collected and matured into MII phase in our culture medium for 24 h. Subsequently, levels of ROS, SOD, MDA, and LDH were tested in OC group and non-OC group using either microplate assays or chemical fluorescence techniques. Finally, a total of 2432 immature oocytes obtained from controlled ovarian hyperstimulation (COH) cycles underwent in vitro maturation (IVM) in culture media with or without oil supplementation (1380 and 1052 oocytes, respectively), followed by insemination and embryo culture. The IVM prognostic indicators were assessed and compared between OC group and non-OC group.

Results

There were no statistically significant differences in pH and osmolality values between the OC group and the non-OC group. However, the concentrations of melatonin, FSH, E2, and hCG in non-OC group were significantly elevated in the non-OC group compared to the OC group. Regarding oxidative stress parameters, levels of ROS, MDA, and LDH in the culture medium of the non-OC group were lower than those in the OC group, while SOD levels were higher in the non-OC group. Furthermore, the clinical outcome assessments revealed significantly higher rates of maturation, cleavage, and blastocyst formation in the non-OC group compared to the OC group, with no discernible difference in fertilization rates between the two groups.

Conclusion

Omission of mineral oil overlay enhances hormone bioavailability, diminishes oxidative stress, and significantly improves oocyte maturation and blastocyst formation rates in IVM culture.