Scholarlink

Background <p>Allergic rhinitis (AR), a major subtype of rhinitis, is characterized by dysregulated apoptosis in the nasal mucosa, a process intrinsically correlated with its inflammatory pathology. The aim of this study was to identify pan-apoptosis-related genes with diagnostic and therapeutic relevance in AR.</p> Methods <p>We analyzed AR-related datasets (GSE75011 and GSE50223) from Gene expression Omnibus (GEO) database. Differentially expressed genes (DEGs) from GSE75011 were intersected with pan-apoptosis-related genes identified by WGCNA. Biomarkers closely associated with AR progression were screened applying LASSO and Recursive Feature Elimination (RFE). Functional enrichment was assessed via GSEA, and single-sample GSEA (ssGSEA) was utilized to analyze the association between immune cell infiltration and the biomarkers. In vitro assays were carried out to further elucidate the role of H1FX in an AR model.</p> Results <p>DEGs were mainly enriched in IL-17 signaling pathway and MAPK signaling pathway. H1FX was identified as a key pan-apoptosis-related biomarker, with an AUC &gt; 0.7 in ROC analysis. GSEA revealed differential enrichment of VEGF signaling pathway, MAPK signaling pathway, and Spliceosome and HALLMARK_G2M_CHECKPOINT between high and low expression groups of H1FX. ssGSEA analysis showed that H1FX expression was positively linked to Activated B cells, Central memory CD4 T cells, and significantly negatively correlated with Activated CD4 T cells in AR. Cellular assays confirmed that H1FX regulated proliferation and apoptosis in the AR model.</p> Conclusion <p>Integrating computational analyses and experimental validation, we identified H1FX as a pivotal regulator of pan-apoptosis and inflammatory responses in AR, highlighting its potential as a diagnostic biomarker and therapeutic target.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

H1FX as a novel biomarker linking pan-apoptosis to immune dysregulation in allergic rhinitis

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年1月31日

查看全文
  • Yisen Liang,
  • Zhuangyan Mai,
  • Rui Li,
  • Ying Liu,
  • Jiabin Zhan

摘要

Background

Allergic rhinitis (AR), a major subtype of rhinitis, is characterized by dysregulated apoptosis in the nasal mucosa, a process intrinsically correlated with its inflammatory pathology. The aim of this study was to identify pan-apoptosis-related genes with diagnostic and therapeutic relevance in AR.

Methods

We analyzed AR-related datasets (GSE75011 and GSE50223) from Gene expression Omnibus (GEO) database. Differentially expressed genes (DEGs) from GSE75011 were intersected with pan-apoptosis-related genes identified by WGCNA. Biomarkers closely associated with AR progression were screened applying LASSO and Recursive Feature Elimination (RFE). Functional enrichment was assessed via GSEA, and single-sample GSEA (ssGSEA) was utilized to analyze the association between immune cell infiltration and the biomarkers. In vitro assays were carried out to further elucidate the role of H1FX in an AR model.

Results

DEGs were mainly enriched in IL-17 signaling pathway and MAPK signaling pathway. H1FX was identified as a key pan-apoptosis-related biomarker, with an AUC > 0.7 in ROC analysis. GSEA revealed differential enrichment of VEGF signaling pathway, MAPK signaling pathway, and Spliceosome and HALLMARK_G2M_CHECKPOINT between high and low expression groups of H1FX. ssGSEA analysis showed that H1FX expression was positively linked to Activated B cells, Central memory CD4 T cells, and significantly negatively correlated with Activated CD4 T cells in AR. Cellular assays confirmed that H1FX regulated proliferation and apoptosis in the AR model.

Conclusion

Integrating computational analyses and experimental validation, we identified H1FX as a pivotal regulator of pan-apoptosis and inflammatory responses in AR, highlighting its potential as a diagnostic biomarker and therapeutic target.