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<p>This study established a mouse model of oxidative stress induced by D-galactose to investigate the effects of <i>Pediococcus pentosaceus</i> CQFP202425 (PP-CQFP202425) on hepatic oxidative stress status and exercise endurance. The exercise capacity of the mice was evaluated using an exhaustive treadmill running test. Serum and liver tissue-related indices were measured using assay kits, and the pathological morphology of liver tissues was observed via hematoxylin-eosin (H&amp;E) staining. Furthermore, quantitative polymerase chain reaction (qPCR) was employed to determine the mRNA expression levels of specific genes in liver tissue, gastrocnemius muscle tissue, and intestinal contents. The results indicated that, compared with the model group, PP-CQFP202425 intervention significantly prolonged the exhaustive running time in mice; reduced the serum levels of lactic acid (LA), creatine kinase (CK), and lipopolysaccharide (LPS); increased the reserves of hepatic glycogen (HG) and muscle glycogen (MG); and concurrently lowered the levels of tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), and reactive oxygen species (ROS). The results also showed that liver tissue sections revealed PP-CQFP202425 alleviated pathological damage induced by oxidative stress. Further molecular analysis demonstrated that PP-CQFP202425 upregulated the mRNA expression of superoxide dismutase 1 (SOD1), SOD2, catalase (CAT), and glutathione peroxidase 4 (GPx4) in both the liver and gastrocnemius muscle of mice with oxidative stress-induced exhaustion. In liver tissue, it also increased the mRNA expression of AMP-activated protein kinase (AMPK) and the mammalian target of rapamycin (mTOR). Analysis of intestinal contents indicated that PP-CQFP202425 increased the mRNA expression of <i>Firmicutes</i> and <i>Bifidobacterium</i> in the model mice, while decreasing the expression of <i>Bacteroidetes</i>. It also significantly elevated the expression of lactic acid bacteria, reaching levels even higher than those in the normal group. These findings suggest that PP-CQFP202425 can mitigate oxidative damage and enhance exercise endurance.</p>

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Effects of Pediococcus pentosaceus CQFP202425 on regulating oxidative stress and exercise performance

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年5月28日

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  • Yan Wang,
  • Zhenfeng Ding

摘要

This study established a mouse model of oxidative stress induced by D-galactose to investigate the effects of Pediococcus pentosaceus CQFP202425 (PP-CQFP202425) on hepatic oxidative stress status and exercise endurance. The exercise capacity of the mice was evaluated using an exhaustive treadmill running test. Serum and liver tissue-related indices were measured using assay kits, and the pathological morphology of liver tissues was observed via hematoxylin-eosin (H&E) staining. Furthermore, quantitative polymerase chain reaction (qPCR) was employed to determine the mRNA expression levels of specific genes in liver tissue, gastrocnemius muscle tissue, and intestinal contents. The results indicated that, compared with the model group, PP-CQFP202425 intervention significantly prolonged the exhaustive running time in mice; reduced the serum levels of lactic acid (LA), creatine kinase (CK), and lipopolysaccharide (LPS); increased the reserves of hepatic glycogen (HG) and muscle glycogen (MG); and concurrently lowered the levels of tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), and reactive oxygen species (ROS). The results also showed that liver tissue sections revealed PP-CQFP202425 alleviated pathological damage induced by oxidative stress. Further molecular analysis demonstrated that PP-CQFP202425 upregulated the mRNA expression of superoxide dismutase 1 (SOD1), SOD2, catalase (CAT), and glutathione peroxidase 4 (GPx4) in both the liver and gastrocnemius muscle of mice with oxidative stress-induced exhaustion. In liver tissue, it also increased the mRNA expression of AMP-activated protein kinase (AMPK) and the mammalian target of rapamycin (mTOR). Analysis of intestinal contents indicated that PP-CQFP202425 increased the mRNA expression of Firmicutes and Bifidobacterium in the model mice, while decreasing the expression of Bacteroidetes. It also significantly elevated the expression of lactic acid bacteria, reaching levels even higher than those in the normal group. These findings suggest that PP-CQFP202425 can mitigate oxidative damage and enhance exercise endurance.