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<p>Sarcopenia starts around age 40, with individuals losing more than 30% of muscle mass by age 70. Due to the absence of approved pharmacological treatments, current management primarily relies on exercise and protein supplementations. <i>Schizonepeta tenuifolia</i> is a medicinal plant commonly found in Korea, China, Japan and is traditionally used for headaches, colds and allergies. However, its preventive effect against sarcopenia have not yet been explored. This study to investigate whether the ethanol extract of <i>Schizonepeta tenuifolia</i> (DKB138) enhances muscle strength in a dexamethasone (DEX)-muscle atrophy mice model. The preventive effects of DKB138 against muscle atrophy were evaluated in DEX-induced models both in vitro and in vivo. In vitro, DEX-induced myotubes were assessed for cell viability to determine cytoprotective effects. In vivo, a DEX-induced muscle atrophy mouse model was used evaluate muscle strength and functional performance. Using UPLC and comparison with reference standards, seven components of DKB138 were identified. DKB138 effectively prevented DEX-induced death in C2C12 skeletal muscle cells. In vivo, mice treated with DKB138 exhibited 28.5% increase in grip strength (***<i>p</i> &lt; 0.001) and a threefold significant increase in running distance (**<i>p</i> &lt; 0.01) compared to the DEX-induced mice. Additionally, DKB138 treatment resulted in downregulation of Murf-1 and Atrogin mRNA while upregulating of MyoD mRNA expression. These finding demonstrate that DKB138 protects against DEX-induced muscle atrophy by suppressing muscle degradation factors and enhancing muscle strength. These results suggest that DKB138 has potential as functional food against muscle dysfunction and atrophy.</p> Graphical Abstract <p></p>

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Schizonepeta tenuifolia alleviated muscle weakness in dexamethasone-induced muscle atrophy mouse model

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年10月30日

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  • Young Sook Kim,
  • Heung Joo Yuk,
  • Jin Hong Jang,
  • Dong-Seon Kim

摘要

Sarcopenia starts around age 40, with individuals losing more than 30% of muscle mass by age 70. Due to the absence of approved pharmacological treatments, current management primarily relies on exercise and protein supplementations. Schizonepeta tenuifolia is a medicinal plant commonly found in Korea, China, Japan and is traditionally used for headaches, colds and allergies. However, its preventive effect against sarcopenia have not yet been explored. This study to investigate whether the ethanol extract of Schizonepeta tenuifolia (DKB138) enhances muscle strength in a dexamethasone (DEX)-muscle atrophy mice model. The preventive effects of DKB138 against muscle atrophy were evaluated in DEX-induced models both in vitro and in vivo. In vitro, DEX-induced myotubes were assessed for cell viability to determine cytoprotective effects. In vivo, a DEX-induced muscle atrophy mouse model was used evaluate muscle strength and functional performance. Using UPLC and comparison with reference standards, seven components of DKB138 were identified. DKB138 effectively prevented DEX-induced death in C2C12 skeletal muscle cells. In vivo, mice treated with DKB138 exhibited 28.5% increase in grip strength (***p < 0.001) and a threefold significant increase in running distance (**p < 0.01) compared to the DEX-induced mice. Additionally, DKB138 treatment resulted in downregulation of Murf-1 and Atrogin mRNA while upregulating of MyoD mRNA expression. These finding demonstrate that DKB138 protects against DEX-induced muscle atrophy by suppressing muscle degradation factors and enhancing muscle strength. These results suggest that DKB138 has potential as functional food against muscle dysfunction and atrophy.

Graphical Abstract