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Background <p><i>Staphylococcus argenteus</i> belongs to the <i>Staphylococcus aureus</i> complex and causes similar disease. Methicillin-resistant <i>S. argenteus</i> (MRSArg) is rare in the Netherlands. We describe the first hospital outbreak and its management.</p> Methods <p>After a healthcare worker linked to a patient with MRSArg bacteremia tested positive, cohort screening of healthcare workers, patients, and family members was performed. Carriers received eradication therapy. Whole-genome multilocus sequence typing (wgMLST) and antimicrobial susceptibility testing were used to characterize isolates and mupirocin resistance.</p> Results <p>Fifteen (2.6%) MRSArg carriers were detected: 9/317 (2.8%) healthcare workers, 4/238 (1.7%) patients, 2/16 (12.5%) family members; age range 22–88 years, median 55 years. Nine (60%) were complicated and six (40%) uncomplicated carriers. After transfer of one initially negative patient to a nursing home, subsequent positivity led to two carriers among nursing home staff. wgMLST confirmed a single outbreak cluster of 15 isolates, all MLST ST2250. The isolates carried <i>mecA</i>, <i>dfrG</i>, and <i>blaZ</i>, but not PVL. Eradication therapy was started in 13/15 (86.7%) persons, with 84.6% (11/13) successful eradication. Six isolates carried a V588F-mutated <i>ileS</i> gene and had mupirocin MICs of 2–32&#xa0;mg/L; nine were sensitive.</p> Conclusions <p>We report the first successfully controlled MRSArg hospital outbreak in the Netherlands. The outbreak strain was clonally related and showed low-level mupirocin resistance linked to a mutated <i>ileS</i> gene. This work supports the importance of infection prevention and control measures that are applied for MRSA are also effective for MRSArg.</p>

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Control of a hospital outbreak with methicillin-resistant Staphylococcus argenteus in The Netherlands, June–September 2023

  • 期刊论文

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  • 发表日期：

    2026年5月30日

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  • Serena Slavenburg,
  • Dennis Souverein,
  • Sandra Witteveen,
  • Esther de Vries,
  • Angela de Haan,
  • Annelot F. Schoffelen,
  • Daan W. Notermans,
  • Bjorn L. Herpers,
  • Antoni P. A. Hendrickx

摘要

Background

Staphylococcus argenteus belongs to the Staphylococcus aureus complex and causes similar disease. Methicillin-resistant S. argenteus (MRSArg) is rare in the Netherlands. We describe the first hospital outbreak and its management.

Methods

After a healthcare worker linked to a patient with MRSArg bacteremia tested positive, cohort screening of healthcare workers, patients, and family members was performed. Carriers received eradication therapy. Whole-genome multilocus sequence typing (wgMLST) and antimicrobial susceptibility testing were used to characterize isolates and mupirocin resistance.

Results

Fifteen (2.6%) MRSArg carriers were detected: 9/317 (2.8%) healthcare workers, 4/238 (1.7%) patients, 2/16 (12.5%) family members; age range 22–88 years, median 55 years. Nine (60%) were complicated and six (40%) uncomplicated carriers. After transfer of one initially negative patient to a nursing home, subsequent positivity led to two carriers among nursing home staff. wgMLST confirmed a single outbreak cluster of 15 isolates, all MLST ST2250. The isolates carried mecA, dfrG, and blaZ, but not PVL. Eradication therapy was started in 13/15 (86.7%) persons, with 84.6% (11/13) successful eradication. Six isolates carried a V588F-mutated ileS gene and had mupirocin MICs of 2–32 mg/L; nine were sensitive.

Conclusions

We report the first successfully controlled MRSArg hospital outbreak in the Netherlands. The outbreak strain was clonally related and showed low-level mupirocin resistance linked to a mutated ileS gene. This work supports the importance of infection prevention and control measures that are applied for MRSA are also effective for MRSArg.