Scholarlink

Background <p>The reference microbiology laboratory of Israel’s National Institute for Antibiotic Resistance and Infection Control has established a national repository of isolates analyzed by Fourier-transform infrared (FTIR) spectroscopy and their spectra. Healthcare institutions send antibiotic-resistant isolates as part of outbreak investigation, periodic nation-wide collection of specific species, or point prevalence studies. Here, we describe the use of a national FTIR repository to detect the emergence and spread of new sequence types and resistance mechanisms.</p> Methods <p>Using FTIR, we produced dendrograms of outbreaks and periodic country-level dendrograms of isolates from selected species. When FTIR identified new clusters that were distinct from previously characterized clusters, they were investigated further by whole genome sequencing.</p> Results <p>FTIR analysis uncovered two clones new to Israel: NDM-5-producing <i>E. coli</i> ST650 harboring a novel plasmid, and NDM-producing<i> K. pneumoniae</i> ST307.</p> Conclusions <p>Establishing regional or national FTIR repositories could serve as a simple and effective tool for early detection of new antibiotic-resistant clones.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Use of a national repository of Fourier-transform infrared spectroscopy spectra enables fast detection of silent outbreaks and prevention of spread of new antibiotic-resistant sequence types

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年4月21日

查看全文
  • Mor N. Lurie-Weinberger,
  • Elizabeth Temkin,
  • Ophir Kastel,
  • Moshe Bechor,
  • Darya Bychenko-Banyas,
  • Reut Efrati-Epchtien,
  • Gabrielle D. Levi,
  • Nadya Rakovitsky,
  • Alona Keren-Paz,
  • Yehuda Carmeli,
  • Orit Golan-Shany,
  • Danielle Keidar-Friedman,
  • Jonathan Lellouche,
  • Chen Levy-Sadot,
  • Lina Haider-Morgan,
  • Ruth Bar Nathan,
  • Ola Salach,
  • Yehudit Schindler,
  • Orna Schwartz Harari,
  • Merav Strauss

摘要

Background

The reference microbiology laboratory of Israel’s National Institute for Antibiotic Resistance and Infection Control has established a national repository of isolates analyzed by Fourier-transform infrared (FTIR) spectroscopy and their spectra. Healthcare institutions send antibiotic-resistant isolates as part of outbreak investigation, periodic nation-wide collection of specific species, or point prevalence studies. Here, we describe the use of a national FTIR repository to detect the emergence and spread of new sequence types and resistance mechanisms.

Methods

Using FTIR, we produced dendrograms of outbreaks and periodic country-level dendrograms of isolates from selected species. When FTIR identified new clusters that were distinct from previously characterized clusters, they were investigated further by whole genome sequencing.

Results

FTIR analysis uncovered two clones new to Israel: NDM-5-producing E. coli ST650 harboring a novel plasmid, and NDM-producing K. pneumoniae ST307.

Conclusions

Establishing regional or national FTIR repositories could serve as a simple and effective tool for early detection of new antibiotic-resistant clones.