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<p>Cytotoxic T lymphocyte (CTL)-mediated platelet destruction represents an important pathogenic mechanism in primary immune thrombocytopenia (ITP) patients. Rho-associated coiled-coil kinase 2 (ROCK2) is an emerging regulator of immune balance, but its role in pathogenic CTL activation in ITP remains undefined. Here, we demonstrated that selective ROCK2 inhibition with KD025 potently suppressed CTL-mediated platelet destruction. In vitro, KD025 treatment of CTLs from ITP patients suppressed key effector functions, reducing degranulation as measured by CD107a expression, diminishing the secretion of cytotoxic molecules such as granzyme B and perforin, and decreasing CTL-platelet conjugate formation, resulting in reduced platelet apoptosis and activation. RNA-sequencing revealed downregulation of cytotoxic and glycolytic programs, with enrichment of JAK-STAT signaling. Mechanistically, KD025 reversed the pathogenic metabolic shift in ITP CTLs by lowering glycolytic flux and restoring mitochondrial respiration, accompanied by decreased STAT3 phosphorylation. IL-6-mediated STAT3 activation largely reversed these effects, indicating a ROCK2-STAT3-dependent mechanism. In vivo, both daily KD025 administration to an active ITP mouse model and transplantation of KD025-pretreated CD8<sup>+</sup> T cells into irradiated <i>Rag1</i><sup><i>−/−</i></sup> mice alleviated CTL-mediated platelet apoptosis and increased platelet counts. Collectively, these findings supported a model in which ROCK2 contributed to STAT3-associated transcriptional and metabolic regulation of CTL pathogenicity, providing a rationale for further preclinical and translational evaluation of ROCK2 inhibition in ITP.</p> Graphical abstract <p></p>

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ROCK2 inhibition suppresses cytotoxic T lymphocyte-mediated platelet destruction in primary immune thrombocytopenia

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年7月20日

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  • Juanjuan Song,
  • Bingjie Ding,
  • Qian Liu,
  • Ao Xie,
  • Mengjuan Li,
  • Xuewen Song,
  • Fengdi Wang,
  • Junxia Hu,
  • Liu Liu,
  • Xinguang Liu,
  • Hu Zhou

摘要

Cytotoxic T lymphocyte (CTL)-mediated platelet destruction represents an important pathogenic mechanism in primary immune thrombocytopenia (ITP) patients. Rho-associated coiled-coil kinase 2 (ROCK2) is an emerging regulator of immune balance, but its role in pathogenic CTL activation in ITP remains undefined. Here, we demonstrated that selective ROCK2 inhibition with KD025 potently suppressed CTL-mediated platelet destruction. In vitro, KD025 treatment of CTLs from ITP patients suppressed key effector functions, reducing degranulation as measured by CD107a expression, diminishing the secretion of cytotoxic molecules such as granzyme B and perforin, and decreasing CTL-platelet conjugate formation, resulting in reduced platelet apoptosis and activation. RNA-sequencing revealed downregulation of cytotoxic and glycolytic programs, with enrichment of JAK-STAT signaling. Mechanistically, KD025 reversed the pathogenic metabolic shift in ITP CTLs by lowering glycolytic flux and restoring mitochondrial respiration, accompanied by decreased STAT3 phosphorylation. IL-6-mediated STAT3 activation largely reversed these effects, indicating a ROCK2-STAT3-dependent mechanism. In vivo, both daily KD025 administration to an active ITP mouse model and transplantation of KD025-pretreated CD8+ T cells into irradiated Rag1−/− mice alleviated CTL-mediated platelet apoptosis and increased platelet counts. Collectively, these findings supported a model in which ROCK2 contributed to STAT3-associated transcriptional and metabolic regulation of CTL pathogenicity, providing a rationale for further preclinical and translational evaluation of ROCK2 inhibition in ITP.

Graphical abstract