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<p>Transferable antimicrobial resistance poses significant threats to public health. This issue is further exacerbated by Lsa homolog-mediated resistance to pleuromutilins, lincosamides, and streptogramin A (PLS<sub>A</sub>). This study aimed to characterize a novel PLS<sub>A</sub> resistance gene, designated Lsa(F), identified in <i>Streptococcus</i> <i>parasuis</i>. The Lsa(F) protein exhibits 41.9–58.7% sequence identity with known Lsa variants and shares structural features typical of the F lineage of the ATP-binding cassette (ABC) protein superfamily that confers ribosome protection. Functional expression of Lsa(F) in <i>Streptococcus</i> <i>suis</i> and <i>Staphylococcus</i> <i>aureus</i> confers resistance to PLS<sub>A</sub>. Although the prevalence of the Lsa(F) gene among <i>S</i>.&#xa0;<i>parasuis</i> isolates was found to be relatively low, database analyses revealed that this gene is primarily present in <i>Streptococcus</i> and <i>Lactococcus</i> species. In <i>Streptococcus</i>, the Lsa(F) gene is predominantly associated with various mobile genetic elements (MGEs), including plasmids, integrative and conjugative elements (ICE), and ICE-derived elements; in <i>Lactococcus</i>, it is primarily located on plasmids and at a specific chromosomal locus. Examination of the genetic context analysis identified the insertion sequence IS<i>Lll1</i> downstream of Lsa(F) on almost all chromosomal MGEs and plasmids, suggesting a potential mechanism facilitating its movement. Notably, our results indicate that the Lsa(F) gene can be transferred from <i>S.&#xa0;parasuis</i> to <i>Streptococcus</i> <i>suis</i>, highlighting a risk of its spread to other pathogens. Therefore, it is necessary to enhance surveillance and epidemiological monitoring of the Lsa(F) gene to reduce the potential public health risks associated with its spread.</p>

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Characterization of a novel gene, Lsa(F), conferring resistance to pleuromutilins, lincosamides and streptogramin A in Streptococcus parasuis

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年7月7日

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  • Xingyang Dai,
  • Paulin Mungongo Mayama,
  • Xiao Liu,
  • Xiao Gao,
  • Jingyan Xu,
  • Xiaoming Wang,
  • Jinhu Huang,
  • Zongfu Wu,
  • Diafuka Saila-Ngita,
  • Liping Wang

摘要

Transferable antimicrobial resistance poses significant threats to public health. This issue is further exacerbated by Lsa homolog-mediated resistance to pleuromutilins, lincosamides, and streptogramin A (PLSA). This study aimed to characterize a novel PLSA resistance gene, designated Lsa(F), identified in Streptococcus parasuis. The Lsa(F) protein exhibits 41.9–58.7% sequence identity with known Lsa variants and shares structural features typical of the F lineage of the ATP-binding cassette (ABC) protein superfamily that confers ribosome protection. Functional expression of Lsa(F) in Streptococcus suis and Staphylococcus aureus confers resistance to PLSA. Although the prevalence of the Lsa(F) gene among S. parasuis isolates was found to be relatively low, database analyses revealed that this gene is primarily present in Streptococcus and Lactococcus species. In Streptococcus, the Lsa(F) gene is predominantly associated with various mobile genetic elements (MGEs), including plasmids, integrative and conjugative elements (ICE), and ICE-derived elements; in Lactococcus, it is primarily located on plasmids and at a specific chromosomal locus. Examination of the genetic context analysis identified the insertion sequence ISLll1 downstream of Lsa(F) on almost all chromosomal MGEs and plasmids, suggesting a potential mechanism facilitating its movement. Notably, our results indicate that the Lsa(F) gene can be transferred from S. parasuis to Streptococcus suis, highlighting a risk of its spread to other pathogens. Therefore, it is necessary to enhance surveillance and epidemiological monitoring of the Lsa(F) gene to reduce the potential public health risks associated with its spread.