Scholarlink

<p>Duck infectious serositis is a septicemic disease caused by the bacterium <i>Riemerella anatipestifer</i> (<i>R. anatipestifer</i>), which affects ducks, geese, turkeys, and other poultry. While outbreaks have been reported worldwide, the exact mechanisms of infection and disease progression remain unclear. Our previous research identified the two-component system PhoPR within the genome of <i>R. anatipestifer</i> and demonstrated its association with the bacterium’s pathogenicity. Through multi-omics analysis, we found that PhoP directly regulates the expression of several genes, including <i>moxR</i>, within the <i>Bacteroides aerotolerance</i> (Bat) operon. However, the function of MoxR in <i>R. anatipestifer</i> has not yet been reported. To investigate the impact of MoxR on the expression of the <i>bat</i> operon and the pathogenicity of <i>R. anatipestifer</i>, we constructed Δ<i>moxR</i> and other derivative strains. Our findings revealed that overexpression of MoxR inhibits the transcription of the <i>bat</i> operon. Conversely, deletion of <i>moxR</i>, along with exposure of <i>R. anatipestifer</i> to thermal or oxidative stress, results in increased transcription levels of the <i>bat</i> operon. By measuring the survival ability of each strain under stress, we discovered that MoxR is closely associated with the resistance of <i>R. anatipestifer</i> to thermal and oxidative stress by influencing the expression of the <i>bat</i> operon. Duckling infection experiments, along with adhesion and invasion assays, showed that deletion of <i>moxR</i> in <i>R. anatipestifer</i> led to decreased pathogenicity, and lower bacterial load in various tissues. Collectively, our findings collectively demonstrate the significant role of MoxR in the anti-stress and pathogenicity of <i>R. anatipestifer</i>, providing new insights into its pathogenic mechanisms.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

MoxR effects as an ATPase on anti-stress and pathogenicity of Riemerella anatipestifer

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年2月17日

查看全文
  • Yang Zhang,
  • Yanhao Zhang,
  • Yushan He,
  • Yarong Hou,
  • Xuedi Li,
  • Xueying Yang,
  • Zutao Zhou,
  • Zili Li

摘要

Duck infectious serositis is a septicemic disease caused by the bacterium Riemerella anatipestifer (R. anatipestifer), which affects ducks, geese, turkeys, and other poultry. While outbreaks have been reported worldwide, the exact mechanisms of infection and disease progression remain unclear. Our previous research identified the two-component system PhoPR within the genome of R. anatipestifer and demonstrated its association with the bacterium’s pathogenicity. Through multi-omics analysis, we found that PhoP directly regulates the expression of several genes, including moxR, within the Bacteroides aerotolerance (Bat) operon. However, the function of MoxR in R. anatipestifer has not yet been reported. To investigate the impact of MoxR on the expression of the bat operon and the pathogenicity of R. anatipestifer, we constructed ΔmoxR and other derivative strains. Our findings revealed that overexpression of MoxR inhibits the transcription of the bat operon. Conversely, deletion of moxR, along with exposure of R. anatipestifer to thermal or oxidative stress, results in increased transcription levels of the bat operon. By measuring the survival ability of each strain under stress, we discovered that MoxR is closely associated with the resistance of R. anatipestifer to thermal and oxidative stress by influencing the expression of the bat operon. Duckling infection experiments, along with adhesion and invasion assays, showed that deletion of moxR in R. anatipestifer led to decreased pathogenicity, and lower bacterial load in various tissues. Collectively, our findings collectively demonstrate the significant role of MoxR in the anti-stress and pathogenicity of R. anatipestifer, providing new insights into its pathogenic mechanisms.