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<p>Neurofilament light chain (Nf-L) is a biomarker for axonal damage in human neurology but is understudied in cattle. With this study we wanted to determine Nf-L stability at two different storage temperatures and Nf-L levels in healthy cattle and the relationship with age, evaluate whether Nf-L holds diagnostic potential for neurological disorders, and whether an association exists between Nf-L in serum and in cerebrospinal fluid (CSF). To do this, we measured Nf-L levels in CSF and serum samples from 49 healthy and 75 sick cattle. Storage at −80&#xa0;°C or −20&#xa0;°C had no impact on Nf-L concentration. Physiological median Nf-L levels were 6.3&#xa0;pg/mL (serum) and 414&#xa0;pg/mL (CSF) in calves and 5.5&#xa0;pg/mL (serum) and 828&#xa0;pg/mL (CSF) in adult cattle. There was no association between Nf-L levels in CSF and calf age (r<sup>2</sup> 0.07, <i>p</i> = 0.13), while a weak association was found for Nf-L in serum (r<sup>2</sup> 0.26, <i>p</i> = 0.01), and a significant association in adult cattle (CSF, r<sup>2</sup> 0.69, <i>p</i> = 0.0001; serum, r<sup>2</sup> 0.68, <i>p</i> = 0.0003). CSF Nf-L levels were higher in samples from animals with degenerative (median Nf-L 49971&#xa0;pg/mL) and infectious central nervous system (CNS) disorders (median Nf-L, age &lt; 2&#xa0;months 8863&#xa0;pg/mL; age 2–12&#xa0;months 17474&#xa0;pg/mL; age 1–6&#xa0;years 3546&#xa0;pg/mL), CNS anomalies and metabolic/toxic disorders. There was a significant association between CSF Nf-L and serum Nf-L in cattle with neurological disorders (r<sup>2</sup> 0.2, <i>p</i> = 0.009). Taken together, these findings suggest the potential of Nf-L as a diagnostic tool in cattle neurology.</p>

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Neurofilament light chain (Nf-L) in cerebrospinal fluid and serum as a potential biomarker in the differential diagnosis of neurological diseases in cattle

  • 期刊论文

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  • 发表日期：

    2025年1月10日

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  • Giorgia Di Muro,
  • Carlotta Tessarolo,
  • Giulia Cagnotti,
  • Alessandra Favole,
  • Sara Ferrini,
  • Ugo Ala,
  • Claudio Bellino,
  • Giuliano Borriello,
  • Marina Gallo,
  • Giulia Iamone,
  • Barbara Iulini,
  • Marzia Pezzolato,
  • Cristina Casalone,
  • Maria Caramelli,
  • Lorenzo Capucci,
  • Patrizia Cavadini,
  • Cristiano Corona,
  • Antonio D’Angelo

摘要

Neurofilament light chain (Nf-L) is a biomarker for axonal damage in human neurology but is understudied in cattle. With this study we wanted to determine Nf-L stability at two different storage temperatures and Nf-L levels in healthy cattle and the relationship with age, evaluate whether Nf-L holds diagnostic potential for neurological disorders, and whether an association exists between Nf-L in serum and in cerebrospinal fluid (CSF). To do this, we measured Nf-L levels in CSF and serum samples from 49 healthy and 75 sick cattle. Storage at −80 °C or −20 °C had no impact on Nf-L concentration. Physiological median Nf-L levels were 6.3 pg/mL (serum) and 414 pg/mL (CSF) in calves and 5.5 pg/mL (serum) and 828 pg/mL (CSF) in adult cattle. There was no association between Nf-L levels in CSF and calf age (r2 0.07, p = 0.13), while a weak association was found for Nf-L in serum (r2 0.26, p = 0.01), and a significant association in adult cattle (CSF, r2 0.69, p = 0.0001; serum, r2 0.68, p = 0.0003). CSF Nf-L levels were higher in samples from animals with degenerative (median Nf-L 49971 pg/mL) and infectious central nervous system (CNS) disorders (median Nf-L, age < 2 months 8863 pg/mL; age 2–12 months 17474 pg/mL; age 1–6 years 3546 pg/mL), CNS anomalies and metabolic/toxic disorders. There was a significant association between CSF Nf-L and serum Nf-L in cattle with neurological disorders (r2 0.2, p = 0.009). Taken together, these findings suggest the potential of Nf-L as a diagnostic tool in cattle neurology.