Scholarlink

Background <p>Neurotmesis, remains a significant clinical challenge due to limited intrinsic regenerative capacity and suboptimal outcomes of current therapies. Mesenchymal stromal cells (MSCs) secretome has emerged as a promising cell-free alternative, providing neurotrophic and immunomodulatory factors to support nerve repair. This study aimed to evaluate the regenerative efficacy of primed adipose-derived MSC secretome in a rat model of sciatic nerve neurotmesis.</p> Methods <p>Human and rat adipose-derived MSCs were cultured and primed under hypoxic and inflammatory conditions. Secretomes were characterized by nanoparticle tracking analysis, proteomics, and total protein quantification. Neurotmesis was induced in Wistar rats, followed by repair with biomaterial alone or combined with human or rat secretome. Functional recovery was assessed by neurophysiological measurements at 6 months. Molecular and morphological regeneration was evaluated.</p> Results <p>Secretome priming enhanced the secretion of neurotrophic factors and immunomodulatory proteins, as confirmed by transcriptomic and proteomic analyses. In vivo, secretome-treated groups showed significantly improved neurophysiological recovery and increased NGF levels. qPCR revealed upregulation of myelination-associated genes in treated nerves. Histological and TEM analyses demonstrated robust axonal regeneration.</p> Conclusions <p>Primed MSC secretome markedly enhances structural and functional recovery after sciatic nerve neurotmesis, supporting its potential as a safe, effective, and scalable cell-free therapy for peripheral nerve repair.</p> Graphical Abstract <p></p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

The role of secretome from mesenchymal stromal cells in promoting nerve regeneration after neurotmesis

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年2月3日

查看全文
  • Yaiza González-Rodríguez,
  • Alejandro Casado-Santos,
  • María Rodríguez-Díaz,
  • Endika Nevado-Sánchez,
  • Francisco Isidro Mesas,
  • Irene Martín-Tamayo,
  • Susana Martínez-Flórez,
  • María Luisa González-Fernández,
  • Jorge Labrador,
  • Vega Villar-Suárez

摘要

Background

Neurotmesis, remains a significant clinical challenge due to limited intrinsic regenerative capacity and suboptimal outcomes of current therapies. Mesenchymal stromal cells (MSCs) secretome has emerged as a promising cell-free alternative, providing neurotrophic and immunomodulatory factors to support nerve repair. This study aimed to evaluate the regenerative efficacy of primed adipose-derived MSC secretome in a rat model of sciatic nerve neurotmesis.

Methods

Human and rat adipose-derived MSCs were cultured and primed under hypoxic and inflammatory conditions. Secretomes were characterized by nanoparticle tracking analysis, proteomics, and total protein quantification. Neurotmesis was induced in Wistar rats, followed by repair with biomaterial alone or combined with human or rat secretome. Functional recovery was assessed by neurophysiological measurements at 6 months. Molecular and morphological regeneration was evaluated.

Results

Secretome priming enhanced the secretion of neurotrophic factors and immunomodulatory proteins, as confirmed by transcriptomic and proteomic analyses. In vivo, secretome-treated groups showed significantly improved neurophysiological recovery and increased NGF levels. qPCR revealed upregulation of myelination-associated genes in treated nerves. Histological and TEM analyses demonstrated robust axonal regeneration.

Conclusions

Primed MSC secretome markedly enhances structural and functional recovery after sciatic nerve neurotmesis, supporting its potential as a safe, effective, and scalable cell-free therapy for peripheral nerve repair.

Graphical Abstract