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Background <p>Increasing clinical and experimental evidence suggests that the upper and lower jaw exhibit pronounced functional and structural disparities that cannot be explained solely by classical anatomical differences. A central, yet unresolved question is whether periodontal ligament stem cells (PDLSCs) from these distinct regions display site-specific molecular signaling patterns in response to mechanical stress. We propose that PDLSCs derived from maxillary and mandibular regions activate distinct kinase signaling networks upon mechanical stimulation, thereby establishing a jaw-specific mechanobiological fingerprint.</p> Methods <p>PDLSCs were isolated from seven healthy donor teeth and exposed to a static compressive force of 2&#xa0;g/cm<sup>2</sup>. Kinase activity was profiled using a high-throughput PamChip<sup>®</sup> peptide arrays that target both protein tyrosine kinases (PTKs) and serine/threonine kinases (STKs). Downstream pathway enrichment analyses were conducted using the Wikipathways, Gene Ontology (GO), KEGG, and Enriched pathways databases.</p> Results <p>Mechanical stimulation induced distinct kinase activation signatures depending on jaw origin. Maxillary PDLSCs displayed a predominance of STK activation, while mandibular cells showed a relative reduction in PTK signaling. Only two PTKs and four STKs were consistently regulated across both regions, supporting the presence of a region-specific mechanotransduction profile.</p> Conclusion <p>These findings support our hypothesis that localized differences in kinase signaling may constitute a molecular basis for the clinically observed jaw-specific phenomena, such as heterogeneous orthodontic tooth movement and alveolar bone remodeling. Jaw-dependent mechanotransduction pathways can therefore be considered key determinants of periodontal biology and may provide a basis for follow-up studies aimed at enabling the development of personalized orthodontic and regenerative strategies.</p>

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Jaw-specific differential kinase activity profiles in human periodontal ligament stem cells under mechanical compression

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年11月7日

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  • Christian Niederau,
  • Sanne L. Maas,
  • Emiel P. C. van der Vorst,
  • Leon J. Schurgers,
  • Yang Shi,
  • Frank Hölzle,
  • Michael Wolf,
  • Rogerio B. Craveiro

摘要

Background

Increasing clinical and experimental evidence suggests that the upper and lower jaw exhibit pronounced functional and structural disparities that cannot be explained solely by classical anatomical differences. A central, yet unresolved question is whether periodontal ligament stem cells (PDLSCs) from these distinct regions display site-specific molecular signaling patterns in response to mechanical stress. We propose that PDLSCs derived from maxillary and mandibular regions activate distinct kinase signaling networks upon mechanical stimulation, thereby establishing a jaw-specific mechanobiological fingerprint.

Methods

PDLSCs were isolated from seven healthy donor teeth and exposed to a static compressive force of 2 g/cm2. Kinase activity was profiled using a high-throughput PamChip® peptide arrays that target both protein tyrosine kinases (PTKs) and serine/threonine kinases (STKs). Downstream pathway enrichment analyses were conducted using the Wikipathways, Gene Ontology (GO), KEGG, and Enriched pathways databases.

Results

Mechanical stimulation induced distinct kinase activation signatures depending on jaw origin. Maxillary PDLSCs displayed a predominance of STK activation, while mandibular cells showed a relative reduction in PTK signaling. Only two PTKs and four STKs were consistently regulated across both regions, supporting the presence of a region-specific mechanotransduction profile.

Conclusion

These findings support our hypothesis that localized differences in kinase signaling may constitute a molecular basis for the clinically observed jaw-specific phenomena, such as heterogeneous orthodontic tooth movement and alveolar bone remodeling. Jaw-dependent mechanotransduction pathways can therefore be considered key determinants of periodontal biology and may provide a basis for follow-up studies aimed at enabling the development of personalized orthodontic and regenerative strategies.