<p>Stem cells from the apical papilla (SCAPs) represent a unique population of mesenchymal stem cells (MSCs) located in the apical papilla of immature permanent teeth. These cells exhibit essential MSC characteristics, including specific marker expression, self-renewal, proliferation, migration, multipotent differentiation, and immunosuppressive properties. Additionally, SCAPs secrete bioactive factors that promote tissue regeneration, making them promising candidates for stem cell-based therapies. Their regenerative potential encompasses diverse applications in dentistry, bone repair, neural regeneration, and vascular engineering, thereby positioning SCAPs as a versatile tool in regenerative medicine. However, challenges such as phenotypic instability during long-term culture and inconsistent regenerative outcomes impede their clinical translation. Addressing these challenges is crucial for advancing the clinical application of SCAPs. This review examines the advantages and therapeutic applications of SCAPs, identifies barriers to their clinical implementation, and highlights opportunities for optimizing their efficacy. By synthesizing current knowledge and proposing future research directions, this work aims to facilitate the development of SCAP-based strategies for tissue repair, bridging the gap between laboratory research and clinical practice.</p> Graphical Abstract <p></p>