A validated restriction enzyme ddPCR cg05575921 (AHRR) assay to accurately assess smoking exposure

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发表日期：

2024年3月25日

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Sandra Fitzgerald,
Basharat Bhat,
Cristin Print,
Gregory T. Jones

摘要

Background & Methods

In this study, a novel restriction enzyme (RE) digestion-based droplet digital polymerase chain reaction (ddPCR) assay was designed for cg005575921 within the AHRR gene body and compared with matching results obtained by bisulfite conversion (BIS) ddPCR and Illumina DNA methylation array.

Results

The RE ddPCR cg05575921 assay appeared concordant with BIS ddPCR (r² = 0.94, P < 0.0001) and, when compared with the Illumina array, had significantly better smoking status classification performance for current versus never smoked (AUC 0.96 versus 0.93, P < 0.04) and current versus ex-smoker (AUC 0.88 versus 0.83, P < 0.04) comparisons.

Conclusions

The RE ddPCR cg05575921 assay accurately predicts smoking status and could be a useful component of ‘precision-medicine’ chronic disease risk screening tools.