Scholarlink

Objectives <p>Fresh vegetables can harbor diverse bacteria, including <i>Pseudomonas</i> spp. In routine diagnostics, isolates are often identified by MALDI-TOF MS, but novel or unreferenced taxa can be misidentified. Here, we report the WGS of <i>Pseudomonas</i> sp. strain PK-RTE-24, initially assigned by MALDI-TOF MS to a known species. The aim was to generate genomic data that clarify the taxonomic assignment of PK-RTE-24 that is not linked to any described species and to highlight the discrepancy between MALDI-TOF MS identification and WGS analysis.</p> Data description <p>PK-RTE-24 was isolated from ready-to-eat lettuce during screening for extended-spectrum β-lactamase (ESBL)-producing bacteria. MALDI-TOF MS reported <i>Pseudomonas koreensis.</i> Using Illumina and Oxford Nanopore technologies a complete 5,847,647-bp genome, with 60.04% GC content, and encoding 5,285 predicted genes, was assembled. Phylogenomic analysis and average nucleotide identity showed that PK-RTE-24 did not match any described <i>Pseudomonas</i> sp. and fell below the species threshold, grouping within an environment-associated clade. Antimicrobials testing revealed β-lactams non-susceptibility, consistent with a chromosomally-encoded class C β-lactamase (AmpC) gene (1,167-bp). The 388 amino-acid protein shared 96.9% identity with an AmpC from <i>Pseudomonas</i> sp. RG1. The PK-RTE-24’s genome sequence provides valuable data for classifying environment-associated <i>Pseudomonas</i> spp. and elucidating β-lactam resistance in non-clinical strains.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Complete genome sequence of Pseudomonas sp. PK-RTE-24 isolated from lettuce

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年5月11日

查看全文
  • Chahrazed Belhout,
  • Claudia Aldeia,
  • Andrea Endimiani

摘要

Objectives

Fresh vegetables can harbor diverse bacteria, including Pseudomonas spp. In routine diagnostics, isolates are often identified by MALDI-TOF MS, but novel or unreferenced taxa can be misidentified. Here, we report the WGS of Pseudomonas sp. strain PK-RTE-24, initially assigned by MALDI-TOF MS to a known species. The aim was to generate genomic data that clarify the taxonomic assignment of PK-RTE-24 that is not linked to any described species and to highlight the discrepancy between MALDI-TOF MS identification and WGS analysis.

Data description

PK-RTE-24 was isolated from ready-to-eat lettuce during screening for extended-spectrum β-lactamase (ESBL)-producing bacteria. MALDI-TOF MS reported Pseudomonas koreensis. Using Illumina and Oxford Nanopore technologies a complete 5,847,647-bp genome, with 60.04% GC content, and encoding 5,285 predicted genes, was assembled. Phylogenomic analysis and average nucleotide identity showed that PK-RTE-24 did not match any described Pseudomonas sp. and fell below the species threshold, grouping within an environment-associated clade. Antimicrobials testing revealed β-lactams non-susceptibility, consistent with a chromosomally-encoded class C β-lactamase (AmpC) gene (1,167-bp). The 388 amino-acid protein shared 96.9% identity with an AmpC from Pseudomonas sp. RG1. The PK-RTE-24’s genome sequence provides valuable data for classifying environment-associated Pseudomonas spp. and elucidating β-lactam resistance in non-clinical strains.