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Objective <p>The aim of this study is to characterize a new <i>Dhillonvirus</i> bacteriophage that is lytic against <i>E. coli</i> strains, particularly <i>E. coli</i> O157:H7. Methods used for characterization included bacteriophage isolation, purification, bacteriophage morphology, host range test, one-step growth curve, whole-genome sequencing and analysis, and preliminary determination of depolymerase activity.</p> Results <p>The complete genome sequence of <i>Escherichia</i> phage vB EcoS UDS3lw consists of double-stranded DNA, with a genome length of 44,071 base pairs and GC content of 54.67%. UDS3lw did not contain bacterial virulence genes, such as <i>stx</i>, or lysogenic genes in the phage genome. The phylogenetic analysis at the nucleotide level revealed that UDS3lw shared a close evolutionary relationship with <i>Escherichia</i> phages SM S22 and SSL-2009a, belonging to the <i>Dhillonvirus</i> genus. UDS3lw has a narrow host spectrum against <i>E. coli</i> O157:H7 and some non-pathogenic <i>E. coli</i> strains. Despite a small burst size of 2.4 PFU/cell on <i>E. coli</i> O157:H7 (RM9995), UDS3lw encodes a tail fiber protein (ORF 31) with depolymerase activity against non-pathogenic <i>E. coli</i> and most <i>E. coli</i> O157 strains. The findings provide insights into the genetic and biological diversity of the <i>E. coli-</i>infecting phage UDS3lw, which exhibits depolymerase activity and holds promise for further development as an anti-biofilm agent.</p>

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Characterization of a newly isolated Dhillonvirus bacteriophage vB_EcoS-UDS3lw against Escherichia coli O157:H7

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年5月16日

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  • Yen-Te Liao,
  • Abigail R. Arellano,
  • Yujie Zhang,
  • Vivian C.H. Wu

摘要

Objective

The aim of this study is to characterize a new Dhillonvirus bacteriophage that is lytic against E. coli strains, particularly E. coli O157:H7. Methods used for characterization included bacteriophage isolation, purification, bacteriophage morphology, host range test, one-step growth curve, whole-genome sequencing and analysis, and preliminary determination of depolymerase activity.

Results

The complete genome sequence of Escherichia phage vB EcoS UDS3lw consists of double-stranded DNA, with a genome length of 44,071 base pairs and GC content of 54.67%. UDS3lw did not contain bacterial virulence genes, such as stx, or lysogenic genes in the phage genome. The phylogenetic analysis at the nucleotide level revealed that UDS3lw shared a close evolutionary relationship with Escherichia phages SM S22 and SSL-2009a, belonging to the Dhillonvirus genus. UDS3lw has a narrow host spectrum against E. coli O157:H7 and some non-pathogenic E. coli strains. Despite a small burst size of 2.4 PFU/cell on E. coli O157:H7 (RM9995), UDS3lw encodes a tail fiber protein (ORF 31) with depolymerase activity against non-pathogenic E. coli and most E. coli O157 strains. The findings provide insights into the genetic and biological diversity of the E. coli-infecting phage UDS3lw, which exhibits depolymerase activity and holds promise for further development as an anti-biofilm agent.